Worldwide diversity of klebsiella pneumoniae that produces β-lactamase bla_KPC-2 Gene

Klebsiella pneumoniae isolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional β-lactamase content. They harbored a naturally chromosome-encoded bla gene (bla_SHV-1 [12.5%], bla_SHV-11 [68.7%], or bla_OKP-A/B [18.8%]) and several acquired and plasmid-encoded genes (bla_TEM-1 [81.3%], bla_CTX-M-2 [31.3%], bla_CTX-M-12 [12.5%], bla_CTX-M-15 [18.7%], and bla_OXA-9 [37.5%]). The bla_KPC-2 gene was always associated with 1 of the Tn4401 isoforms (a, b, or c). Tn4401 was inserted on different-sized plasmids that belonged to different incompatibility groups. Several bla_KPC-containing K. pneumoniae clones were found: 9 different pulsotypes with 1 major (sequence type 258) and 7 minor distinct allelic profiles. Different clones harboring different plasmids but having identical genetic structure, Tn4401, could be at the origin of the worldwide spread of this emerging resistance gene.