Repeated, Long-Term Cycling of Putative Stem Cells between Niches in a Basal Chordate

Yuval Rinkevich, Ayelet Voskoboynik, Amalia Rosner, Claudette Rabinowitz, Guy Paz, Matan Oren, Jacob Douek, Gilad Alfassi, Elizabeth Moiseeva, Katherine J. Ishizuka, Karla J. Palmeri, Irving L. Weissman, Buki Rinkevich

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

89 ציטוטים ‏(Scopus)

תקציר

The mechanisms that sustain stem cells are fundamental to tissue maintenance. Here, we identify " cell islands" (CIs) as a niche for putative germ and somatic stem cells in Botryllus schlosseri, a colonial chordate that undergoes weekly cycles of death and regeneration. Cells within CIs express markers associated with germ and somatic stem cells and gene products that implicate CIs as signaling centers for stem cells. Transplantation of CIs induced long-term germline and somatic chimerism, demonstrating self-renewal and pluripotency of CI cells. Cell labeling and in vivo time-lapse imaging of CI cells reveal waves of migrations from degrading CIs into developing buds, contributing to soma and germline development. Knockdown of cadherin, which is highly expressed within CIs, elicited the migration of CI cells to circulation. Piwi knockdown resulted in regeneration arrest. We suggest that repeated trafficking of stem cells allows them to escape constraints imposed by the niche, enabling self-preservation throughout life.

שפה מקוריתאנגלית
עמודים (מ-עד)76-88
מספר עמודים13
כתב עתDevelopmental Cell
כרך24
מספר גיליון1
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 14 ינו׳ 2013
פורסם באופן חיצוניכן

טביעת אצבע

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