TY - JOUR
T1 - Protective effect of free-radical scavengers on corneal endothelial damage in phacoemulsification
AU - Nemet, Arie Y.
AU - Assia, Ehud I.
AU - Meyerstein, Dan
AU - Meyerstein, Naomi
AU - Gedanken, Aharon
AU - Topaz, Morris
N1 - Funding Information:
Funded in part by a grant from the Clair and Amedee Martier Institute for the Study of Blindness and Visual Disorders, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
PY - 2007/2
Y1 - 2007/2
N2 - Purpose: To examine the role of the water-soluble antioxidants glutathione and ascorbic acid in the irrigating solution on corneal endothelial cells following exposure to high-intensity ultrasound energy. Setting: Goldschleger Eye Research Institute, Sheba Medical Center, Tel-Aviv University, Tel Aviv, Israel. Methods: Thirty-two rabbit eyes were subjected to prolonged exposure to the phacoemulsification device in the anterior chamber. The eyes were divided into 4 groups that differed only in the composition of the irrigating solution applied to the eyes: balanced salt solution (BSS) BSS Plus BSS containing additional soluble components including glutathione, BSS with 10-3 M of oxidized glutathione (GSSG), and BSS with 10-2 M of ascorbic acid. Specular microscopy was performed preoperatively and 1 week after surgery. Results: The BSS group exhibited the highest endothelial cell loss (19.3%), followed by the BSS Plus group (10.6%), the GSSG group (5.2%), and the ascorbic acid group (0.9%). An overall difference was found between the groups (F = 11.046, P<.0001), and all groups demonstrated a statistically significant difference from the control BSS group (P<.02, P = .001, and P<.0001, respectively). Conclusions: Damage to the cornea is largely due to the free radicals generated by high-intensity ultrasound energy during phacoemulsification. Adding the antioxidants ascorbic acid and GSSG to the irrigation solution significantly reduced the endothelial corneal cell damage. Ascorbic acid in the concentration of 10-2 M had the highest protective effect; thus, it should be evaluated for clinical use.
AB - Purpose: To examine the role of the water-soluble antioxidants glutathione and ascorbic acid in the irrigating solution on corneal endothelial cells following exposure to high-intensity ultrasound energy. Setting: Goldschleger Eye Research Institute, Sheba Medical Center, Tel-Aviv University, Tel Aviv, Israel. Methods: Thirty-two rabbit eyes were subjected to prolonged exposure to the phacoemulsification device in the anterior chamber. The eyes were divided into 4 groups that differed only in the composition of the irrigating solution applied to the eyes: balanced salt solution (BSS) BSS Plus BSS containing additional soluble components including glutathione, BSS with 10-3 M of oxidized glutathione (GSSG), and BSS with 10-2 M of ascorbic acid. Specular microscopy was performed preoperatively and 1 week after surgery. Results: The BSS group exhibited the highest endothelial cell loss (19.3%), followed by the BSS Plus group (10.6%), the GSSG group (5.2%), and the ascorbic acid group (0.9%). An overall difference was found between the groups (F = 11.046, P<.0001), and all groups demonstrated a statistically significant difference from the control BSS group (P<.02, P = .001, and P<.0001, respectively). Conclusions: Damage to the cornea is largely due to the free radicals generated by high-intensity ultrasound energy during phacoemulsification. Adding the antioxidants ascorbic acid and GSSG to the irrigation solution significantly reduced the endothelial corneal cell damage. Ascorbic acid in the concentration of 10-2 M had the highest protective effect; thus, it should be evaluated for clinical use.
UR - http://www.scopus.com/inward/record.url?scp=33846688645&partnerID=8YFLogxK
U2 - 10.1016/j.jcrs.2006.10.031
DO - 10.1016/j.jcrs.2006.10.031
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C2 - 17276276
AN - SCOPUS:33846688645
SN - 0886-3350
VL - 33
SP - 310
EP - 315
JO - Journal of Cataract and Refractive Surgery
JF - Journal of Cataract and Refractive Surgery
IS - 2
ER -