TY - JOUR
T1 - Preparation of stable standards of biological tissues for laser ablation analysis
AU - Sela, H.
AU - Karpas, Z.
AU - Cohen, H.
AU - Zakon, Y.
AU - Zeiri, Y.
N1 - Funding Information:
The authors would like to acknowledge the support from the Geological Survey of Israel (GSI), Jerusalem for letting us use their facilities. Special thank given to Dr. Ludwik Halicz for his help and support throughout the project.
PY - 2011/10/1
Y1 - 2011/10/1
N2 - A novel method for preparation of durable standards of biological samples for quantitative analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed. Rat brain tissue samples were ground and then spiked by aqueous solutions containing controlled amounts of trace metals of interest. The spiked rat brain samples were then encapsulated in a sol-gel matrix produced by tetraethyl orthosilicate (TEOS). The spiked standards were prepared by addition of 0-50 μg g-1 of eight elements (Li, Co, Cr, Cu, Fe, Mn, Pb and Zn) to the biological samples. The correlation coefficients of the calibration curves for all elements tested were better than 0.97. Carbon-13, found in all biological samples, was used as an internal standard to correct for variations in the amount of material ablated and carried to the ICP-MS plasma torch, a common problem in LA-ICP-MS analysis. The results of analysis of the standards showed very good repeatability and homogeneity as well as long room temperature shelf life.
AB - A novel method for preparation of durable standards of biological samples for quantitative analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed. Rat brain tissue samples were ground and then spiked by aqueous solutions containing controlled amounts of trace metals of interest. The spiked rat brain samples were then encapsulated in a sol-gel matrix produced by tetraethyl orthosilicate (TEOS). The spiked standards were prepared by addition of 0-50 μg g-1 of eight elements (Li, Co, Cr, Cu, Fe, Mn, Pb and Zn) to the biological samples. The correlation coefficients of the calibration curves for all elements tested were better than 0.97. Carbon-13, found in all biological samples, was used as an internal standard to correct for variations in the amount of material ablated and carried to the ICP-MS plasma torch, a common problem in LA-ICP-MS analysis. The results of analysis of the standards showed very good repeatability and homogeneity as well as long room temperature shelf life.
KW - Biological samples
KW - Laser ablation ICP-MS
KW - Sol-gel matrix
KW - Standards
KW - Trace elements
UR - https://www.scopus.com/pages/publications/80052963452
U2 - 10.1016/j.ijms.2011.01.022
DO - 10.1016/j.ijms.2011.01.022
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AN - SCOPUS:80052963452
SN - 1387-3806
VL - 307
SP - 142
EP - 148
JO - International Journal of Mass Spectrometry
JF - International Journal of Mass Spectrometry
IS - 1-3
ER -