Near-infrared squaraine dyes for fluorescence enhanced surface assay

Evgenia G. Matveeva; Ewald A. Terpetschnig; Megan Stevens; Leonid Patsenker; Olga S. Kolosova; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1016/j.dyepig.2008.04.008

Near-infrared squaraine dyes for fluorescence enhanced surface assay

Evgenia G. Matveeva, Ewald A. Terpetschnig, Megan Stevens, Leonid Patsenker, Olga S. Kolosova, Zygmunt Gryczynski, Ignacy Gryczynski

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

18 ציטוטים ‏(Scopus)

תקציר

Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

שפה מקורית	אנגלית
עמודים (מ-עד)	41-46
מספר עמודים	6
כתב עת	Dyes and Pigments
כרך	80
מספר גיליון	1
מזהי עצם דיגיטלי (DOIs)	https://doi.org/10.1016/j.dyepig.2008.04.008
סטטוס פרסום	פורסם - ינו׳ 2009
פורסם באופן חיצוני	כן

גישה למסמך

10.1016/j.dyepig.2008.04.008

קבצים וקישורים אחרים

Link to publication in Scopus

פורמט ציטוט ביבליוגרפי

@article{5c6784a4218444adb076428519e61f3c,

title = "Near-infrared squaraine dyes for fluorescence enhanced surface assay",

abstract = "Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.",

keywords = "Enhanced fluorescence immunoassays, Near-infrared fluorescent squaraine dyes, Sensors, Seta dyes, Silver island films, Total internal reflection",

author = "Matveeva, {Evgenia G.} and Terpetschnig, {Ewald A.} and Megan Stevens and Leonid Patsenker and Kolosova, {Olga S.} and Zygmunt Gryczynski and Ignacy Gryczynski",

note = "Funding Information: This work was supported by Texas Emerging Technologies Fund grant and by NIH HG 004364 and NSF (DBI-0649889). ",

year = "2009",

month = jan,

doi = "10.1016/j.dyepig.2008.04.008",

language = "אנגלית",

volume = "80",

pages = "41--46",

journal = "Dyes and Pigments",

issn = "0143-7208",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Near-infrared squaraine dyes for fluorescence enhanced surface assay

AU - Matveeva, Evgenia G.

AU - Terpetschnig, Ewald A.

AU - Stevens, Megan

AU - Patsenker, Leonid

AU - Kolosova, Olga S.

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

N1 - Funding Information: This work was supported by Texas Emerging Technologies Fund grant and by NIH HG 004364 and NSF (DBI-0649889).

PY - 2009/1

Y1 - 2009/1

N2 - Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

AB - Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

KW - Enhanced fluorescence immunoassays

KW - Near-infrared fluorescent squaraine dyes

KW - Sensors

KW - Seta dyes

KW - Silver island films

KW - Total internal reflection

UR - http://www.scopus.com/inward/record.url?scp=51249114771&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2008.04.008

DO - 10.1016/j.dyepig.2008.04.008

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AN - SCOPUS:51249114771

SN - 0143-7208

VL - 80

SP - 41

EP - 46

JO - Dyes and Pigments

JF - Dyes and Pigments

IS - 1

ER -

Near-infrared squaraine dyes for fluorescence enhanced surface assay

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