תקציר
Gaussian network model (GNM) modes of motion are calculated to a dataset of hemoglobin (Hb) structures and modes with dynamics similarity to the T state are multiply aligned. The sole criterion for the alignment is the mode shape itself and not sequence or structural similarity. Standard deviation (SD) of the GNM value score along the alignment is calculated, regions with high SD are defined as dynamically variable. The analysis shows that the α1β1/α2β2 interface is a dynamically variable region but not the α1β2/α2β1 and the α1α2/β1β2 interfaces. The results are in accordance with the T → R2 transition of Hb. We suggest that dynamically variable regions are regions that are likely to undergo structural change in the protein upon binding, conformational transition, or any other relevant chemical event. The represented technique of multiple dynamics-based alignment of modes is novel and may offer a new insight in proteins' dynamics to function relation.
שפה מקורית | אנגלית |
---|---|
עמודים (מ-עד) | 2097-2105 |
מספר עמודים | 9 |
כתב עת | Proteins: Structure, Function and Bioinformatics |
כרך | 82 |
מספר גיליון | 9 |
מזהי עצם דיגיטלי (DOIs) | |
סטטוס פרסום | פורסם - ספט׳ 2014 |