Levels of mRNA coding for α-, β-, and γ-synuclein in the brains of newborn, juvenile, and adult rats

Ewa Malatynska, Albert Pinhasov, Jeffrey Crooke, Daniel Horowitz, Douglas E. Brenneman, Sergey E. Ilyin

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

13 ציטוטים ‏(Scopus)

תקציר

Synucleins are proteins known for their malfunction in a group of illnesses called synucleopathies, which includes Alzheimer's and Parkinson's disease. To learn more about the role of synucleins in the CNS, we have studied levels of message coding for α-, β-, and γ-synuclein using quantitative RT-PCR. Levels of synuclein mRNAs were studied in the cerebral cortex (left and right, anterior and posterior), hippocampus, striatum, and cerebellum, obtained from 5-d-old (newborn), 1-mo (juvenile)-, and 6-, and 9-mo (adult)-old rats. The mRNA levels for all synucleins varied significantly among structures. The rank order of mRNA levels in different structures was cortex = hippocampus > striatum > cerebellum for α-synuclein; cortex > hippocampus = cerebellum > striatum for β-synuclein; and hippocampus = striatum > cortex = cerebellum for γ-synuclein. There was significant effect of age for mRNAlevels for all synucleins. The dynamics of these changes were different depending on type of synuclein and brain structure. Levels of mRNA for α-synuclein were significantly reduced with age in all structures except hippocampus. For β- and γ-synuclein, levels increased significantly only in the cerebral cortex and only from 5 d to 1 mo of age. In contrast, γ-synuclein levels in the cerebellum were very high at 5 d and significantly reduced at 1 mo of age. The revealed pattern and dynamics of changes in the levels of mRNA coding for synucleins would support the conclusion for an important role of these molecules during development and the aging process.

שפה מקוריתאנגלית
עמודים (מ-עד)269-277
מספר עמודים9
כתב עת Journal of Molecular Neuroscience
כרך29
מספר גיליון3
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - יולי 2006
פורסם באופן חיצוניכן

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