TY - JOUR
T1 - Laboratory evaluation of different agar media for isolation of carbapenem-resistant Acinetobacter spp
AU - Moran-Gilad, J.
AU - Adler, A.
AU - Schwartz, D.
AU - Navon-Venezia, S.
AU - Carmeli, Y.
N1 - Publisher Copyright:
© 2014, Springer-Verlag Berlin Heidelberg.
PY - 2014/10/19
Y1 - 2014/10/19
N2 - The optimal method for surveillance of carbapenem-resistant Acinetobacter spp. (CRAB) is unknown. A collection of CRAB strains (n = 42), carbapenem-susceptible strains (CSAB), and non-Acinetobacter strains (n = 18) was used to evaluate six laboratory surveillance methods: MacConkey (MAC), MAC + 1 μg/ml imipenem (MAC-IPM), minimal salts agar + 1 % acetate (MSA), MSA with IPM disk (MSA-IPM), CHROMagarKPC, and CHROMagar Acinetobacter with CR102 (CHROMAcineto). CHROMAcineto was 100 % sensitive and specific. CHROMagarKPC and MAC-IPM were highly sensitive (>95 %), but their specificity was substantially hampered by the breakthrough growth of CSAB. MSA was unsuitable for CRAB detection. CHROMAcineto is a promising medium for CRAB detection and warrants further clinical evaluation.
AB - The optimal method for surveillance of carbapenem-resistant Acinetobacter spp. (CRAB) is unknown. A collection of CRAB strains (n = 42), carbapenem-susceptible strains (CSAB), and non-Acinetobacter strains (n = 18) was used to evaluate six laboratory surveillance methods: MacConkey (MAC), MAC + 1 μg/ml imipenem (MAC-IPM), minimal salts agar + 1 % acetate (MSA), MSA with IPM disk (MSA-IPM), CHROMagarKPC, and CHROMagar Acinetobacter with CR102 (CHROMAcineto). CHROMAcineto was 100 % sensitive and specific. CHROMagarKPC and MAC-IPM were highly sensitive (>95 %), but their specificity was substantially hampered by the breakthrough growth of CSAB. MSA was unsuitable for CRAB detection. CHROMAcineto is a promising medium for CRAB detection and warrants further clinical evaluation.
UR - http://www.scopus.com/inward/record.url?scp=84911007396&partnerID=8YFLogxK
U2 - 10.1007/s10096-014-2159-y
DO - 10.1007/s10096-014-2159-y
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C2 - 24865248
AN - SCOPUS:84911007396
SN - 0934-9723
VL - 33
SP - 1909
EP - 1913
JO - European Journal of Clinical Microbiology and Infectious Diseases
JF - European Journal of Clinical Microbiology and Infectious Diseases
IS - 11
ER -