Kinetics of erythrocyte swelling and membrane hole formation in hypotonic media

Alexander Pribush, Dan Meyerstein, Naomi Meyerstein

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

31 ציטוטים ‏(Scopus)

תקציר

Red blood cell (RBC) swelling and membrane hole formation in hypotonic external media were studied by measuring the time-dependent capacitance, C, and the conductance, G, in the beginning of the β-dispersion range. At high and moderate osmolarities of the external solution the capacitance reaches a steady-state whereas at low osmolarities it reveals a biphasic kinetics. Examination of RBC suspensions exposed to different concentrations of HgCl2 demonstrates that water transport through mercury-sensitive water channel controls RBC swelling. Unlike the capacitance, an increase in the conductance to a stationary level is observed after a certain delay. A comparison of G(t) curves recorded for the suspensions of the intact cells and those treated with cytochalasin B or glutaraldehyde demonstrates the significant effect of the membrane viscoelasticity on the pore formation. It is shown that the stretched membrane of completely swollen RBC retains its integrity for a certain time, termed as the membrane lifetime, tmemb. Therefore, the resistivity of RBCs to a certain osmotic shock may be quantified by the distribution function of RBC(tmemb).

שפה מקוריתאנגלית
עמודים (מ-עד)119-132
מספר עמודים14
כתב עתBiochimica et Biophysica Acta - Biomembranes
כרך1558
מספר גיליון2
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 1 פבר׳ 2002

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Kinetics of erythrocyte swelling and membrane hole formation in hypotonic media'. יחד הם יוצרים טביעת אצבע ייחודית.

פורמט ציטוט ביבליוגרפי