Development of sensitive methods for the detection of myobacteria by DNA probes

Galia Rahav; Shlomo Sela; Hervé Bercovier

doi:10.1016/0378-1097(90)90339-R

Development of sensitive methods for the detection of myobacteria by DNA probes

Galia Rahav, Shlomo Sela, Hervé Bercovier

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

1 ציטוט ‏(Scopus)

תקציר

A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

שפה מקורית	אנגלית
עמודים (מ-עד)	29-33
מספר עמודים	5
כתב עת	FEMS Microbiology Letters
כרך	72
מספר גיליון	1-2
מזהי עצם דיגיטלי (DOIs)	https://doi.org/10.1016/0378-1097(90)90339-R
סטטוס פרסום	פורסם - אוק׳ 1990
פורסם באופן חיצוני	כן

גישה למסמך

10.1016/0378-1097(90)90339-R

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Link to publication in Scopus

פורמט ציטוט ביבליוגרפי

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title = "Development of sensitive methods for the detection of myobacteria by DNA probes",

abstract = "A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.",

keywords = "DNA probes, DNA-DNA hybridization, Mycobacteria, Sonication",

author = "Galia Rahav and Shlomo Sela and Herv{\'e} Bercovier",

note = "Funding Information: We would like to thank R. David-Pour for her excellent technical assistance. This work was in part funded by a grant from the Hebrew University and Hadassah Hospital common research fund.",

year = "1990",

month = oct,

doi = "10.1016/0378-1097(90)90339-R",

language = "אנגלית",

volume = "72",

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journal = "FEMS Microbiology Letters",

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T1 - Development of sensitive methods for the detection of myobacteria by DNA probes

AU - Rahav, Galia

AU - Sela, Shlomo

AU - Bercovier, Hervé

N1 - Funding Information: We would like to thank R. David-Pour for her excellent technical assistance. This work was in part funded by a grant from the Hebrew University and Hadassah Hospital common research fund.

PY - 1990/10

Y1 - 1990/10

N2 - A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

AB - A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

KW - DNA probes

KW - DNA-DNA hybridization

KW - Mycobacteria

KW - Sonication

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JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1-2

ER -

Development of sensitive methods for the detection of myobacteria by DNA probes

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