Determination of coproporphyrinogen III oxidase activity in bacteria

Rivka Mamet; Ram Scharf; Nathan Keller; Yoram Zimmels; Nili Schoenfeld

doi:10.1016/0167-7012(94)90067-1

Determination of coproporphyrinogen III oxidase activity in bacteria

Rivka Mamet
, Ram Scharf
, Nathan Keller
, Yoram Zimmels
, Nili Schoenfeld

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

3 ציטוטים ‏(Scopus)

תקציר

A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

שפה מקורית	אנגלית
עמודים (מ-עד)	173-178
מספר עמודים	6
כתב עת	Journal of Microbiological Methods
כרך	19
מספר גיליון	3
מזהי עצם דיגיטלי (DOIs)	https://doi.org/10.1016/0167-7012(94)90067-1
סטטוס פרסום	פורסם - מרץ 1994
פורסם באופן חיצוני	כן

גישה למסמך

10.1016/0167-7012(94)90067-1

קבצים וקישורים אחרים

Link to publication in Scopus

פורמט ציטוט ביבליוגרפי

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title = "Determination of coproporphyrinogen III oxidase activity in bacteria",

abstract = "A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.",

keywords = "Coproporphyrinogen III oxidase, E. coli, HPLC",

author = "Rivka Mamet and Ram Scharf and Nathan Keller and Yoram Zimmels and Nili Schoenfeld",

year = "1994",

month = mar,

doi = "10.1016/0167-7012(94)90067-1",

language = "אנגלית",

volume = "19",

pages = "173--178",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Determination of coproporphyrinogen III oxidase activity in bacteria

AU - Mamet, Rivka

AU - Scharf, Ram

AU - Keller, Nathan

AU - Zimmels, Yoram

AU - Schoenfeld, Nili

PY - 1994/3

Y1 - 1994/3

N2 - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

AB - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

KW - Coproporphyrinogen III oxidase

KW - E. coli

KW - HPLC

UR - https://www.scopus.com/pages/publications/0028348848

U2 - 10.1016/0167-7012(94)90067-1

DO - 10.1016/0167-7012(94)90067-1

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AN - SCOPUS:0028348848

SN - 0167-7012

VL - 19

SP - 173

EP - 178

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Determination of coproporphyrinogen III oxidase activity in bacteria

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