Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

J. R. Hutson; K. Aleksa; F. Pragst; G. Koren

doi:10.1016/j.jchromb.2008.10.056

Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

J. R. Hutson, K. Aleksa, F. Pragst, G. Koren

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

37 ציטוטים ‏(Scopus)

תקציר

Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

שפה מקורית	אנגלית
עמודים (מ-עד)	8-12
מספר עמודים	5
כתב עת	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
כרך	877
מספר גיליון	1-2
מזהי עצם דיגיטלי (DOIs)	https://doi.org/10.1016/j.jchromb.2008.10.056
סטטוס פרסום	פורסם - 1 ינו׳ 2009
פורסם באופן חיצוני	כן

גישה למסמך

10.1016/j.jchromb.2008.10.056

קבצים וקישורים אחרים

Link to publication in Scopus

טביעת אצבע

להלן מוצגים תחומי המחקר של הפרסום 'Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry'. יחד הם יוצרים טביעת אצבע ייחודית.

פורמט ציטוט ביבליוגרפי

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title = "Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry",

abstract = "Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.",

keywords = "Biomarker, Fatty acid ethyl esters, Gas chromatography, Headspace-solid-phase microextraction, Meconium",

author = "Hutson, {J. R.} and K. Aleksa and F. Pragst and G. Koren",

note = "Funding Information: JRH is supported by the University of Toronto{\textquoteright}s Comprehensive Research Experience for Medical Students Summer Studentship and a CIHR MD/PhD Studentship. GK is the holder of the Ivey Chair in Molecular Toxicology, The Department of Medicine, University of Western Ontario.",

year = "2009",

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doi = "10.1016/j.jchromb.2008.10.056",

language = "אנגלית",

volume = "877",

pages = "8--12",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Hutson, JR, Aleksa, K, Pragst, F & Koren, G 2009, 'Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, כרך 877, מס' 1-2, עמודים 8-12. https://doi.org/10.1016/j.jchromb.2008.10.056

Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry. / Hutson, J. R.; Aleksa, K.; Pragst, F. et al.
ב-: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, כרך 877, מס' 1-2, 01.01.2009, עמוד 8-12.

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

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T1 - Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

AU - Hutson, J. R.

AU - Aleksa, K.

AU - Pragst, F.

AU - Koren, G.

N1 - Funding Information: JRH is supported by the University of Toronto’s Comprehensive Research Experience for Medical Students Summer Studentship and a CIHR MD/PhD Studentship. GK is the holder of the Ivey Chair in Molecular Toxicology, The Department of Medicine, University of Western Ontario.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

AB - Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

KW - Biomarker

KW - Fatty acid ethyl esters

KW - Gas chromatography

KW - Headspace-solid-phase microextraction

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