Binding of acetaldehyde to human and guinea pig placentae in vitro

Marjie L. Hard, Umar Iqbal, James F. Brien, Gideon Koren

פרסום מחקרי: פרסום בכתב עתמאמרביקורת עמיתים

3 ציטוטים ‏(Scopus)

תקציר

Background. Significant interindividual variability exists following maternal alcohol consumption; not all children born to alcoholic women manifest the symptoms associated with foetal alcohol spectrum disorder (FASD). Objective. To investigate the potential role of the placenta as a source of variability by determining if interindividual variability exists in the binding of acetaldehyde to human placenta. Methods. Acetaldehyde was added to ten different human placental homogenates and subjected to equilibrium dialysis. Homogenates of placentae obtained from guinea pigs chronically exposed to ethanol throughout gestation were also dialysed in the presence of acetaldehyde to look for alterations in binding after chronic alcohol exposure. Nonlinear least-squares regression analysis was used to characterize the binding system involved. Results. It was found that the amount of acetaldehyde bound to human placentae varied by as much as 3-fold among placentae. The binding profile of acetaldehyde was characterized as a two site binding system (Ka1 = 9.8 × 105 ± 0.7 × 105 l/mol, N1 = 1.1 × 10-8 ± 0.7 × 10-8 mol/g tissue; Ka2 = 1.6 × 104 ± 0.9 × 104 l/mol, N2 = 1.7 × 10-7 ± 0.4 × 10-7 mol/g tissue). Chronic alcohol exposure had no effect on the degree of acetaldehyde binding. Conclusion. This previously unidentified source of variability may partially explain why some foetuses are adversely affected by prenatal alcohol exposure while others are not.

שפה מקוריתאנגלית
עמודים (מ-עד)149-154
מספר עמודים6
כתב עתPlacenta
כרך24
מספר גיליון2-3
מזהי עצם דיגיטלי (DOIs)
סטטוס פרסוםפורסם - 2003
פורסם באופן חיצוניכן

טביעת אצבע

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