A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes

Guy Patchornik; Amnon Albeck

doi:10.1021/bc050361+

A general approach for antibody purification utilizing [Protein A-Catechol:Fe³⁺] macro-complexes

Guy Patchornik
, Amnon Albeck

פרסום מחקרי: פרסום בכתב עת › מאמר › ביקורת עמיתים

4 ציטוטים ‏(Scopus)

תקציר

A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe³⁺ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe ³⁺]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe³⁺] insoluble macro-complex.

שפה מקורית	אנגלית
עמודים (מ-עד)	258-260
מספר עמודים	3
כתב עת	Bioconjugate Chemistry
כרך	17
מספר גיליון	2
מזהי עצם דיגיטלי (DOIs)	https://doi.org/10.1021/bc050361+
סטטוס פרסום	פורסם - 2006
פורסם באופן חיצוני	כן

גישה למסמך

10.1021/bc050361+

קבצים וקישורים אחרים

Link to publication in Scopus

פורמט ציטוט ביבליוגרפי

@article{70bc25d890214b6e8bf1913762dab330,

title = "A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes",

abstract = "A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe 3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80\%) and high purity (>95\%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.",

author = "Guy Patchornik and Amnon Albeck",

year = "2006",

doi = "10.1021/bc050361+",

language = "אנגלית",

volume = "17",

pages = "258--260",

journal = "Bioconjugate Chemistry",

issn = "1043-1802",

publisher = "American Chemical Society",

number = "2",

}

TY - JOUR

T1 - A general approach for antibody purification utilizing [Protein A-Catechol:Fe3+] macro-complexes

AU - Patchornik, Guy

AU - Albeck, Amnon

PY - 2006

Y1 - 2006

N2 - A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe 3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.

AB - A general platform for antibody purification utilizing free nonimmobilized Protein A modified with the strong metal chelator catechol (ProA-CAT) and Fe3+ ions is presented. The mechanism of purification requires formation and precipitation of macro-complexes composed of [ProA-CAT:IgG:Fe 3+]. Target IgGs are eluted directly from the precipitates (i.e. pellets) at pH 3 in high yields (71-80%) and high purity (>95%), without dissociating the [ProA-CAT:Fe3+] insoluble macro-complex.

UR - https://www.scopus.com/pages/publications/33645471037

U2 - 10.1021/bc050361+

DO - 10.1021/bc050361+

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 16536453

AN - SCOPUS:33645471037

SN - 1043-1802

VL - 17

SP - 258

EP - 260

JO - Bioconjugate Chemistry

JF - Bioconjugate Chemistry

IS - 2