Vertically Aligned Nitrogen-Doped Carbon Nanotube Carpet Electrodes: Highly Sensitive Interfaces for the Analysis of Serum from Patients with Inflammatory Bowel Disease

Qian Wang, Palaniappan Subramanian, Alex Schechter, Eti Teblum, Reut Yemini, Gilbert Daniel Nessim, Alina Vasilescu, Musen Li, Rabah Boukherroub, Sabine Szunerits

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The number of patients suffering from inflammatory bowel disease (IBD) is increasing worldwide. The development of noninvasive tests that are rapid, sensitive, specific, and simple would allow preventing patient discomfort, delay in diagnosis, and the follow-up of the status of the disease. Herein, we show the interest of vertically aligned nitrogen-doped carbon nanotube (VA-NCNT) electrodes for the required sensitive electrochemical detection of lysozyme in serum, a protein that is up-regulated in IBD. To achieve selective lysozyme detection, biotinylated lysozyme aptamers were covalently immobilized onto the VA-NCNTs. Detection of lysozyme in serum was achieved by measuring the decrease in the peak current of the Fe(CN)63-/4- redox couple by differential pulse voltammetry upon addition of the analyte. We achieved a detection limit as low as 100 fM with a linear range up to 7 pM, in line with the required demands for the determination of lysozyme level in patients suffering from IBD. We attained the sensitive detection of biomarkers in clinical samples of healthy patients and individuals suffering from IBD and compared the results to a classical turbidimetric assay. The results clearly indicate that the newly developed sensor allows for a reliable and efficient analysis of lysozyme in serum. (Chemical Equation Presented).

Original languageEnglish
Pages (from-to)9600-9609
Number of pages10
JournalACS applied materials & interfaces
Volume8
Issue number15
DOIs
StatePublished - 20 Apr 2016

Keywords

  • aptamers
  • differential pulse voltammetry
  • inflammatory bowel disease
  • lysozyme
  • nitrogen-doped vertically aligned carbon nanotubes
  • sensing

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