TNF-α and TNFR1 responses to recovery therapies following acute resistance exercise

Jeremy R. Townsend; Jay R. Hoffman; Maren S. Fragala; Adam R. Jajtner; Adam M. Gonzalez; Adam J. Wells; Gerald T. Mangine; David H. Fukuda; Jeffrey R. Stout

doi:10.3389/fphys.2015.00048

TNF-α and TNFR1 responses to recovery therapies following acute resistance exercise

Jeremy R. Townsend, Jay R. Hoffman, Maren S. Fragala, Adam R. Jajtner, Adam M. Gonzalez, Adam J. Wells, Gerald T. Mangine, David H. Fukuda, Jeffrey R. Stout

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The purpose of this investigation was to compare the effect of two commonly used therapeutic modalities (a) neuromuscular electrical stimulation (NMES) and (b) cold water immersion (CWI) on circulating tumor necrosis factor alpha (TNF-a) and monocyte TNF-a receptor (TNFR1) expression following intense acute resistance exercise and subsequent recovery. Thirty (n = 30) resistance trained men (22.5 ± 2.7 y) performed an acute heavy resistance exercise protocol on three consecutive days followed by one of three recovery methods (CON, NMES, and CWI). Circulating TNF-a levels were assayed and TNFR1 expression on CD14+ monocytes was measured by flow cytometry measured PRE, immediately post (IP), 30-min post (30M), 24 h post (24H), and 48 h post (48H) exercise. Circulating TNF-a was elevated at IP (p = 0.001) and 30M (p = 0.005) and decreased at 24H and 48H recovery from IP in CON (p = 0.015) and CWI (p = 0.011). TNF-a did not significantly decrease from IP during recovery in NMES. TNFR1 expression was elevated (p < 0.001) at 30M compared to PRE and all other time points. No significant differences between groups were observed in TNFR1 expression. During recovery (24H, 48H) from muscle damaging exercise, NMES treatment appears to prevent the decline in circulating TNF-a observed during recovery in those receiving no treatment or CWI.

Original language	English
Pages (from-to)	48
Number of pages	1
Journal	Frontiers in Physiology
Volume	6
Issue number	FEB
DOIs	https://doi.org/10.3389/fphys.2015.00048
State	Published - 2015
Externally published	Yes

Keywords

Athletic training
Cytokines
Immune function
Muscle damage
TNF-α

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10.3389/fphys.2015.00048

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title = "TNF-α and TNFR1 responses to recovery therapies following acute resistance exercise",

abstract = "The purpose of this investigation was to compare the effect of two commonly used therapeutic modalities (a) neuromuscular electrical stimulation (NMES) and (b) cold water immersion (CWI) on circulating tumor necrosis factor alpha (TNF-a) and monocyte TNF-a receptor (TNFR1) expression following intense acute resistance exercise and subsequent recovery. Thirty (n = 30) resistance trained men (22.5 ± 2.7 y) performed an acute heavy resistance exercise protocol on three consecutive days followed by one of three recovery methods (CON, NMES, and CWI). Circulating TNF-a levels were assayed and TNFR1 expression on CD14+ monocytes was measured by flow cytometry measured PRE, immediately post (IP), 30-min post (30M), 24 h post (24H), and 48 h post (48H) exercise. Circulating TNF-a was elevated at IP (p = 0.001) and 30M (p = 0.005) and decreased at 24H and 48H recovery from IP in CON (p = 0.015) and CWI (p = 0.011). TNF-a did not significantly decrease from IP during recovery in NMES. TNFR1 expression was elevated (p < 0.001) at 30M compared to PRE and all other time points. No significant differences between groups were observed in TNFR1 expression. During recovery (24H, 48H) from muscle damaging exercise, NMES treatment appears to prevent the decline in circulating TNF-a observed during recovery in those receiving no treatment or CWI.",

keywords = "Athletic training, Cytokines, Immune function, Muscle damage, TNF-α",

author = "Townsend, {Jeremy R.} and Hoffman, {Jay R.} and Fragala, {Maren S.} and Jajtner, {Adam R.} and Gonzalez, {Adam M.} and Wells, {Adam J.} and Mangine, {Gerald T.} and Fukuda, {David H.} and Stout, {Jeffrey R.}",

note = "Publisher Copyright: {\textcopyright} 2015 Townsend, Hoffman, Fragala, Jajtner, Gonzalez, Wells, Mangine, Fukuda and Stout.",

year = "2015",

doi = "10.3389/fphys.2015.00048",

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AU - Townsend, Jeremy R.

AU - Hoffman, Jay R.

AU - Fragala, Maren S.

AU - Jajtner, Adam R.

AU - Gonzalez, Adam M.

AU - Wells, Adam J.

AU - Mangine, Gerald T.

AU - Fukuda, David H.

AU - Stout, Jeffrey R.

PY - 2015

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N2 - The purpose of this investigation was to compare the effect of two commonly used therapeutic modalities (a) neuromuscular electrical stimulation (NMES) and (b) cold water immersion (CWI) on circulating tumor necrosis factor alpha (TNF-a) and monocyte TNF-a receptor (TNFR1) expression following intense acute resistance exercise and subsequent recovery. Thirty (n = 30) resistance trained men (22.5 ± 2.7 y) performed an acute heavy resistance exercise protocol on three consecutive days followed by one of three recovery methods (CON, NMES, and CWI). Circulating TNF-a levels were assayed and TNFR1 expression on CD14+ monocytes was measured by flow cytometry measured PRE, immediately post (IP), 30-min post (30M), 24 h post (24H), and 48 h post (48H) exercise. Circulating TNF-a was elevated at IP (p = 0.001) and 30M (p = 0.005) and decreased at 24H and 48H recovery from IP in CON (p = 0.015) and CWI (p = 0.011). TNF-a did not significantly decrease from IP during recovery in NMES. TNFR1 expression was elevated (p < 0.001) at 30M compared to PRE and all other time points. No significant differences between groups were observed in TNFR1 expression. During recovery (24H, 48H) from muscle damaging exercise, NMES treatment appears to prevent the decline in circulating TNF-a observed during recovery in those receiving no treatment or CWI.

AB - The purpose of this investigation was to compare the effect of two commonly used therapeutic modalities (a) neuromuscular electrical stimulation (NMES) and (b) cold water immersion (CWI) on circulating tumor necrosis factor alpha (TNF-a) and monocyte TNF-a receptor (TNFR1) expression following intense acute resistance exercise and subsequent recovery. Thirty (n = 30) resistance trained men (22.5 ± 2.7 y) performed an acute heavy resistance exercise protocol on three consecutive days followed by one of three recovery methods (CON, NMES, and CWI). Circulating TNF-a levels were assayed and TNFR1 expression on CD14+ monocytes was measured by flow cytometry measured PRE, immediately post (IP), 30-min post (30M), 24 h post (24H), and 48 h post (48H) exercise. Circulating TNF-a was elevated at IP (p = 0.001) and 30M (p = 0.005) and decreased at 24H and 48H recovery from IP in CON (p = 0.015) and CWI (p = 0.011). TNF-a did not significantly decrease from IP during recovery in NMES. TNFR1 expression was elevated (p < 0.001) at 30M compared to PRE and all other time points. No significant differences between groups were observed in TNFR1 expression. During recovery (24H, 48H) from muscle damaging exercise, NMES treatment appears to prevent the decline in circulating TNF-a observed during recovery in those receiving no treatment or CWI.

KW - Athletic training

KW - Cytokines

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TNF-α and TNFR1 responses to recovery therapies following acute resistance exercise

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Keywords

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