TY - JOUR
T1 - The dielectric spectroscopy of human red blood cells
T2 - The differentiation of old from fresh cells
AU - David, Marcelo
AU - Levy, Evgeniya
AU - Feldman, Yuri
AU - Ben Ishai, Paul
AU - Zelig, Orly
AU - Yedgar, Saul
AU - Barshtein, Gregory
N1 - Publisher Copyright:
© 2017 Institute of Physics and Engineering in Medicine.
PY - 2017/6/22
Y1 - 2017/6/22
N2 - Objective: The objective of the study was to gauge the effect of storage lesions on the dielectric response of red blood cells (RBC), in particular those processes linked to deformations of the cellular membrane known as the β-dispersion. Approach: The dielectric response of RBC suspensions, exposed to blood-bank cold storage, was studied using time-domain dielectric spectroscopy (TDDS) in the frequency range of 500 kHz up to 1 GHz. The measured dielectric processes are characterized by their dielectric strength (Δϵ) and relaxation time (τ). Changes in the dielectric properties of the RBC suspensions due to storage-related lesions were evaluated. For a quantitative characterization of RBC lesions, we measured the deformability of fresh and stored RBC as expressed by their elongation ratio (ER), which was achieved under a shear stress of 3.0 Pa. Main Result: The results show that the storage of RBC induced a statistically significant decrease of dielectric relaxation times. In addition, a sound correlation between the mean values of ER and the relaxation times was observed (Spearman's correlation coefficient ρ = 0.847). We draw the conclusion that those alterations in the relaxation time are induced by changes in the shape of the RBC that happen during cold-storage. Significance: The evolution of the β-dispersion of RBC opens new possibilities in the blood bank inventory management.
AB - Objective: The objective of the study was to gauge the effect of storage lesions on the dielectric response of red blood cells (RBC), in particular those processes linked to deformations of the cellular membrane known as the β-dispersion. Approach: The dielectric response of RBC suspensions, exposed to blood-bank cold storage, was studied using time-domain dielectric spectroscopy (TDDS) in the frequency range of 500 kHz up to 1 GHz. The measured dielectric processes are characterized by their dielectric strength (Δϵ) and relaxation time (τ). Changes in the dielectric properties of the RBC suspensions due to storage-related lesions were evaluated. For a quantitative characterization of RBC lesions, we measured the deformability of fresh and stored RBC as expressed by their elongation ratio (ER), which was achieved under a shear stress of 3.0 Pa. Main Result: The results show that the storage of RBC induced a statistically significant decrease of dielectric relaxation times. In addition, a sound correlation between the mean values of ER and the relaxation times was observed (Spearman's correlation coefficient ρ = 0.847). We draw the conclusion that those alterations in the relaxation time are induced by changes in the shape of the RBC that happen during cold-storage. Significance: The evolution of the β-dispersion of RBC opens new possibilities in the blood bank inventory management.
KW - aging
KW - dielectric spectroscopy
KW - erythrocytes
KW - red blood cells
KW - storage
UR - http://www.scopus.com/inward/record.url?scp=85020728317&partnerID=8YFLogxK
U2 - 10.1088/1361-6579/aa707a
DO - 10.1088/1361-6579/aa707a
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C2 - 28462905
AN - SCOPUS:85020728317
SN - 0967-3334
VL - 38
SP - 1335
EP - 1348
JO - Physiological Measurement
JF - Physiological Measurement
IS - 7
ER -