The α2/δ subunit of voltage sensitive Ca2+ channels is a single transmembrane extracellular protein which is involved in regulated secretion

Ofer Wiser, Michael Trus, Dror Tobi, Sarah Halevi, Eli Giladi, Daphne Atlas

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The membrane topology of α2/δ subunit was investigated utilizing electrophysiological functional assay and specific anti-α2 antibodies, (a) cRNA encoding a deleted α2/δ subunit was coinjected with α1C subunit of the L-type calcium channel into Xenopus oocytes. The truncated form, lacking the third putative TM domain (α2/δΔTMIII), failed to amplify the expressed inward currents, normally induced by α1C coinjected with intact α2/δ subunit. Western blot analysis of α2/δΔTMIII shows the appearance of a degraded α2 protein and no expression of the full-size two-TM truncated-protein. The improper processing of α2/δΔTMIII suggests that the α2/δ is a single TM domain protein and the TM region is positioned at the δ subunit. (b) External application of anti-α2 antibodies, prepared for an epitope within the alternatively spliced and 'intracellular' region, inhibits depolarization induced secretion in PC12, further supporting an external location of the α2 subunit and establishing δ subunit as the only membrane anchor for the extracellular α2 subunit.

Original languageEnglish
Pages (from-to)15-20
Number of pages6
JournalFEBS Letters
Volume379
Issue number1
DOIs
StatePublished - 22 Jan 1996
Externally publishedYes

Keywords

  • Ca
  • Channel
  • Exocytosis
  • PC12 cell
  • Regulated secretion
  • α2 Subunit
  • δ Subunit

Fingerprint

Dive into the research topics of 'The α2/δ subunit of voltage sensitive Ca2+ channels is a single transmembrane extracellular protein which is involved in regulated secretion'. Together they form a unique fingerprint.

Cite this