Susceptibility of Helicobacter pylori isolates to the antiadhesion activity of a high-molecular-weight constituent of cranberry

Haim Shmuely, Ora Burger, Itzhak Neeman, Jacob Yahav, Zmira Samra, Yaron Niv, Nathan Sharon, Erwin Weiss, Abed Athamna, Miriam Tabak, Itzhak Ofek

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80 Scopus citations

Abstract

The sensitivity of a large number of antibiotic-resistant and nonresistant Helicobacter pylori isolates to the antiadhesion effect of a high-molecular-mass, nondialysable constituent of cranberry juice was tested. Confluent monolayers of gastric cell line in microtiter plate wells were exposed to bacterial suspensions prepared from 83 H. pylori isolates from antibiotic-treated and untreated patients in the presence and absence of the cranberry constituent. Urease assay was used to calculate the percentage of adhesion inhibition. In two thirds of the isolates, adhesion to the gastric cells was inhibited by 0.2 mg/mL of the nondialysable material. There was no relationship between the antiadhesion effect of the cranberry material and metronidazole resistance in isolates from either treated or untreated patients (N = 35). Only 13 isolates (16%) were resistant to both the nondialysable material and metronidazole, and 30 (36%) were resistant to the nondialysable material alone. There was no cross-resistance to the nondialysable material and metronidazole. These data suggest that a combination of antibiotics and a cranberry preparation may improve H. pylori eradication.

Original languageEnglish
Pages (from-to)231-235
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume50
Issue number4
DOIs
StatePublished - Dec 2004
Externally publishedYes

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