TY - JOUR
T1 - Simultaneous determination of lidocaine, prilocaine and the prilocaine metabolite o-toluidine in plasma by high-performance liquid chromatography
AU - Klein, J.
AU - Fernandes, D.
AU - Gazarian, M.
AU - Kent, G.
AU - Koren, G.
N1 - Funding Information:
This study was supported by Astra Pharmaceuticals, Canada.
PY - 1994/4/22
Y1 - 1994/4/22
N2 - An HPLC assay is described for the measurement of prilocaine and lidocaine (components of the local anesthetic cream EMLA) as well as the prilocaine metabolite, o-toluidine, in plasma. The method uses UV detection, is simple, sensitive and most important, only a single 200-μl plasma sample is needed for simultaneous analysis of prilocaine, lidocaine and o-toluidine with a detection limit of 4 ng/ml. The plasma, together with the internal standard (bupivacaine) is extracted with diethyl ether under alkaline conditions, followed by the extraction of the analytes from the organic phase into dilute sulphuric acid. An aliquot of the acid extract is injected onto the HPLC system and the effluent is monitored by a UV detector.
AB - An HPLC assay is described for the measurement of prilocaine and lidocaine (components of the local anesthetic cream EMLA) as well as the prilocaine metabolite, o-toluidine, in plasma. The method uses UV detection, is simple, sensitive and most important, only a single 200-μl plasma sample is needed for simultaneous analysis of prilocaine, lidocaine and o-toluidine with a detection limit of 4 ng/ml. The plasma, together with the internal standard (bupivacaine) is extracted with diethyl ether under alkaline conditions, followed by the extraction of the analytes from the organic phase into dilute sulphuric acid. An aliquot of the acid extract is injected onto the HPLC system and the effluent is monitored by a UV detector.
UR - http://www.scopus.com/inward/record.url?scp=0028274125&partnerID=8YFLogxK
U2 - 10.1016/0378-4347(94)00062-X
DO - 10.1016/0378-4347(94)00062-X
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C2 - 8061836
AN - SCOPUS:0028274125
SN - 0378-4347
VL - 655
SP - 83
EP - 88
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - 1
ER -