TY - JOUR
T1 - Simple measurement of captopril in plasma by high-performance liquid chromatography with ultraviolet detection
AU - Klein, Julia
AU - Colin, Patrick
AU - Scherer, Erick
AU - Levy, Maurice
AU - Koren, Gideon
PY - 1990/1
Y1 - 1990/1
N2 - Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10–1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method.
AB - Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10–1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method.
KW - Captopril
KW - DDPM
KW - High-performance liquid chromatography
KW - Ultraviolet detection
KW - ρ-bromophenacyl bromide
UR - http://www.scopus.com/inward/record.url?scp=0025020044&partnerID=8YFLogxK
U2 - 10.1097/00007691-199001000-00019
DO - 10.1097/00007691-199001000-00019
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C2 - 2406997
AN - SCOPUS:0025020044
SN - 0163-4356
VL - 12
SP - 105
EP - 110
JO - Therapeutic Drug Monitoring
JF - Therapeutic Drug Monitoring
IS - 1
ER -