Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling

Adelina Ovcharenko; Galit Granot; Ofer Shpilberg; Pia Raanani

doi:10.1016/j.leukres.2013.03.010

Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling

Adelina Ovcharenko, Galit Granot, Ofer Shpilberg, Pia Raanani

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Since the introduction of all-. trans-retinoic acid (ATRA) treatment for acute promyelocytic leukemia (APL) there has been increasing concern about extramedullary disease (EMD) progression despite favorable response in the bone marrow. We postulated that ATRA treatment enhances migration and adhesion abilities possibly enabling APL cells to inhabit extramedullary sites. We revealed an increase in adhesion, migration and invasion capabilities of NB4 cells following ATRA treatment. ATRA induced upregulation of Pyk2 mRNA, protein and phosphorylation levels and enhanced Pyk2 interaction with paxillin and vinculin. Pyk2 inhibition resulted in a reduction of NB4 cell adhesion and migration following ATRA treatment. These results indicate that in vitro Pyk2 might function to regulate cell adhesion and motility following ATRA treatment and its upregulated expression may contribute to EMD development in APL patients.

Original language	English
Pages (from-to)	956-962
Number of pages	7
Journal	Leukemia Research
Volume	37
Issue number	8
DOIs	https://doi.org/10.1016/j.leukres.2013.03.010
State	Published - Aug 2013
Externally published	Yes

Keywords

ATRA
Acute promyelocytic leukemia
Adhesion
Extramedullary disease
Migration
Pyk2

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10.1016/j.leukres.2013.03.010

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title = "Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling",

abstract = "Since the introduction of all-. trans-retinoic acid (ATRA) treatment for acute promyelocytic leukemia (APL) there has been increasing concern about extramedullary disease (EMD) progression despite favorable response in the bone marrow. We postulated that ATRA treatment enhances migration and adhesion abilities possibly enabling APL cells to inhabit extramedullary sites. We revealed an increase in adhesion, migration and invasion capabilities of NB4 cells following ATRA treatment. ATRA induced upregulation of Pyk2 mRNA, protein and phosphorylation levels and enhanced Pyk2 interaction with paxillin and vinculin. Pyk2 inhibition resulted in a reduction of NB4 cell adhesion and migration following ATRA treatment. These results indicate that in vitro Pyk2 might function to regulate cell adhesion and motility following ATRA treatment and its upregulated expression may contribute to EMD development in APL patients.",

keywords = "ATRA, Acute promyelocytic leukemia, Adhesion, Extramedullary disease, Migration, Pyk2",

author = "Adelina Ovcharenko and Galit Granot and Ofer Shpilberg and Pia Raanani",

note = "Funding Information: This study was supported by the Van Bits Scholarship donated by the Cancer Research Center, Tel-Aviv University , Israel. ",

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AU - Ovcharenko, Adelina

AU - Granot, Galit

AU - Shpilberg, Ofer

AU - Raanani, Pia

N1 - Funding Information: This study was supported by the Van Bits Scholarship donated by the Cancer Research Center, Tel-Aviv University , Israel.

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AB - Since the introduction of all-. trans-retinoic acid (ATRA) treatment for acute promyelocytic leukemia (APL) there has been increasing concern about extramedullary disease (EMD) progression despite favorable response in the bone marrow. We postulated that ATRA treatment enhances migration and adhesion abilities possibly enabling APL cells to inhabit extramedullary sites. We revealed an increase in adhesion, migration and invasion capabilities of NB4 cells following ATRA treatment. ATRA induced upregulation of Pyk2 mRNA, protein and phosphorylation levels and enhanced Pyk2 interaction with paxillin and vinculin. Pyk2 inhibition resulted in a reduction of NB4 cell adhesion and migration following ATRA treatment. These results indicate that in vitro Pyk2 might function to regulate cell adhesion and motility following ATRA treatment and its upregulated expression may contribute to EMD development in APL patients.

KW - ATRA

KW - Acute promyelocytic leukemia

KW - Adhesion

KW - Extramedullary disease

KW - Migration

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ER -

Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling

Abstract

Keywords

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