Rapid preparation of lambda phage DNA

M. Raikhinstein; I. Hanukoglu

Rapid preparation of lambda phage DNA

M. Raikhinstein
, I. Hanukoglu

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.

Original language	English
Pages (from-to)	108-111
Number of pages	4
Journal	Methods in Molecular and Cellular Biology
Volume	3
Issue number	2
State	Published - 1992
Externally published	Yes

Cite this

@article{54697539a19d4e8ab303bc48d548fe35,

title = "Rapid preparation of lambda phage DNA",

abstract = "A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.",

author = "M. Raikhinstein and I. Hanukoglu",

year = "1992",

language = "אנגלית",

volume = "3",

pages = "108--111",

journal = "Methods in Molecular and Cellular Biology",

issn = "0898-7750",

publisher = "John Wiley and Sons Ltd",

number = "2",

}

TY - JOUR

T1 - Rapid preparation of lambda phage DNA

AU - Raikhinstein, M.

AU - Hanukoglu, I.

PY - 1992

Y1 - 1992

N2 - A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.

AB - A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.

UR - https://www.scopus.com/pages/publications/0026547856

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0026547856

SN - 0898-7750

VL - 3

SP - 108

EP - 111

JO - Methods in Molecular and Cellular Biology

JF - Methods in Molecular and Cellular Biology

IS - 2

ER -

Rapid preparation of lambda phage DNA

Abstract

Other files and links

Fingerprint

Cite this