Abstract
A rapid procedure for the isolation of λ phage DNA is described. The method combines the advantages of direct lysate ultracentrifugation with phage purification on a DEAE-cellulose minicolumn. It can be applied to either liquid or plate lysates on a small or medium scale. The isolated DNA is suitable for restriction and direct sequencing.
| Original language | English |
|---|---|
| Pages (from-to) | 108-111 |
| Number of pages | 4 |
| Journal | Methods in Molecular and Cellular Biology |
| Volume | 3 |
| Issue number | 2 |
| State | Published - 1992 |
| Externally published | Yes |