TY - JOUR
T1 - Radiation damage to the erythrocyte membrane
T2 - The effects of medium and cell concentrations
AU - Pribush, Alexander
AU - Agam, Galila
AU - Yermiahu, Tikva
AU - Dvilansky, Alexander
AU - Meyerstein, Dan
AU - Meyerstein, Naomi
N1 - Funding Information:
This work was supported in part by Ministry of Absorption. We wish to thank Mrs. I. Evens for continuous interest and support.
PY - 1994
Y1 - 1994
N2 - Human erythrocytes suspended in plasma, or in phosphate buffered saline (PBS), were exposed to ionizing radiation. Potassium leakage from irradiated erythrocytes is significantly higher in PBS than in plasma. The potassium leakage decreases when PBS is gradually replaced by plasma. These findings suggest that some of the plasma constituents have radioprotective properties. The potassium leakage per cell is independent of the hematocrit, Hct. The potassium leakage is attributed to the formation of radiation defects in the membrane. Analysis of the effect of radiation dose, plasma and cell concentrations on the product of the number and surface area of the radiation defects indicates that the radiation damage is mainly due to the direct formation of free radicals in the cell membrane. The radioprotective effect of plasma is attributed to surface reactions of these free radicals with plasma constituents adsorbed on the membrane.
AB - Human erythrocytes suspended in plasma, or in phosphate buffered saline (PBS), were exposed to ionizing radiation. Potassium leakage from irradiated erythrocytes is significantly higher in PBS than in plasma. The potassium leakage decreases when PBS is gradually replaced by plasma. These findings suggest that some of the plasma constituents have radioprotective properties. The potassium leakage per cell is independent of the hematocrit, Hct. The potassium leakage is attributed to the formation of radiation defects in the membrane. Analysis of the effect of radiation dose, plasma and cell concentrations on the product of the number and surface area of the radiation defects indicates that the radiation damage is mainly due to the direct formation of free radicals in the cell membrane. The radioprotective effect of plasma is attributed to surface reactions of these free radicals with plasma constituents adsorbed on the membrane.
KW - Erythrocyte membrane
KW - External medium
KW - Hematocrit
KW - Irradiation
KW - Potassium leakage
UR - http://www.scopus.com/inward/record.url?scp=0028500721&partnerID=8YFLogxK
U2 - 10.3109/10715769409056565
DO - 10.3109/10715769409056565
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C2 - 7981785
AN - SCOPUS:0028500721
SN - 1071-5762
VL - 21
SP - 135
EP - 146
JO - Free Radical Research
JF - Free Radical Research
IS - 3
ER -