Quantification of drug release degree in Vivo using antibody-guided, dual-NIR-dye ratiometric system

Ebaston Thankarajan, Suchita Jadhav, Galia Luboshits, Gary Gellerman, Leonid Patsenker

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Fluorescent dyes linked to drug delivery systems provide important real-time information on the efficacy of drug delivery. However, the quantitative monitoring of drug distribution is challenging because of interferences from the biological sample and instrumental setup. To improve quantification of anticancer drug delivery followed by drug release in tumor, we equipped an antibody-drug conjugate (ADC) with a turn-on near-infrared (NIR) dye, sensitive to drug release, and a reference NIR dye. In this study, chlorambucil (CLB) was chosen as a model anticancer drug and Trastuzumab monoclonal antibody specific to Her2 receptors overexpressed in many tumors was taken as the carrier. The advantage of the obtained dual-dye ratiometric system for drug release monitoring was demonstrated in mice model.

Original languageEnglish
Pages (from-to)8265-8272
Number of pages8
JournalAnalytical Chemistry
Volume93
Issue number23
DOIs
StatePublished - 15 Jun 2021

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