Progesterone metabolism in the pineal, brain stem, thalamus and corpus callosum of the female rat

Israel Hanukoglu, Harry J. Karavolas, Robert W. Goy

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Specific brain regions, namely, thalamus, tectum, tegmentum, cerebellum, medulla and pineal, from five proestrous rats were incubated for 30 min with [3H]progesterone. After reverse isotopic dilution analysis, the following metabolites were identified in all incubations by purification to constant specific activity, derivative formation and/or gas liquid chromatography trapping: [3H]5α-pregnane-3, 20-dione (10-20% of the starting substrate except pineal - 0.7%), [3H]3α-hydroxy-5α-pregnan-20-one (1.6-3.8% except for pineal - 0.5%) and [3H]20α-hydroxy-4-pregnen-3-one (0.05-0.11%). Preliminary results from the corpus collosum incubation indicated the presence of the same metabolites. Although some apparent constant specific activities were obtained for 20α-hydroxy-5α-pregnan-3-one and 5β-pregnane-3, 20-dione, the low levels of 3H associated with these steroids did not permit a definitive identification. The results indicate the presence of at least Δ4-steroid 5α-reductase, 3α-hydroxysteroid dehydrogenase and 20α-hydroxysteroid dehydrogenase activities with progesterone as substrate in the brain regions examined.

Original languageEnglish
Pages (from-to)313-324
Number of pages12
JournalBrain Research
Volume125
Issue number2
DOIs
StatePublished - 15 Apr 1977
Externally publishedYes

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