Preparation of stable standards of biological tissues for laser ablation analysis

H. Sela, Z. Karpas, H. Cohen, Y. Zakon, Y. Zeiri

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


A novel method for preparation of durable standards of biological samples for quantitative analysis by laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed. Rat brain tissue samples were ground and then spiked by aqueous solutions containing controlled amounts of trace metals of interest. The spiked rat brain samples were then encapsulated in a sol-gel matrix produced by tetraethyl orthosilicate (TEOS). The spiked standards were prepared by addition of 0-50 μg g-1 of eight elements (Li, Co, Cr, Cu, Fe, Mn, Pb and Zn) to the biological samples. The correlation coefficients of the calibration curves for all elements tested were better than 0.97. Carbon-13, found in all biological samples, was used as an internal standard to correct for variations in the amount of material ablated and carried to the ICP-MS plasma torch, a common problem in LA-ICP-MS analysis. The results of analysis of the standards showed very good repeatability and homogeneity as well as long room temperature shelf life.

Original languageEnglish
Pages (from-to)142-148
Number of pages7
JournalInternational Journal of Mass Spectrometry
Issue number1-3
StatePublished - 1 Oct 2011
Externally publishedYes


  • Biological samples
  • Laser ablation ICP-MS
  • Sol-gel matrix
  • Standards
  • Trace elements


Dive into the research topics of 'Preparation of stable standards of biological tissues for laser ablation analysis'. Together they form a unique fingerprint.

Cite this