TY - JOUR
T1 - Polymer-immobilized photosensitizers for continuous eradication of bacteria
AU - Valkov, Anton
AU - Nakonechny, Faina
AU - Nisnevitch, Marina
PY - 2014/8/25
Y1 - 2014/8/25
N2 - The photosensitizers Rose Bengal (RB) and methylene blue (MB), when immobilized in polystyrene, were found to exhibit high antibacterial activity in a continuous regime. The photosensitizers were immobilized by dissolution in chloroform, together with polystyrene, with further evaporation of the solvent, yielding thin polymeric films. Shallow reservoirs, bottom-covered with these films, were used for constructing continuous-flow photoreactors for the eradication of Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli and wastewater bacteria under illumination with visible white light using a luminescent lamp at a 1.8 mW·cm-2 fluence rate. The bacterial concentration decreased by two to five orders of magnitude in separate reactors with either immobilized RB or MB, as well as in three reactors connected in series, which contained one of the photosensitizers. Bacterial eradication reached more than five orders of magnitude in two reactors connected in series, where the first reactor contained immobilized RB and the second contained immobilized MB.
AB - The photosensitizers Rose Bengal (RB) and methylene blue (MB), when immobilized in polystyrene, were found to exhibit high antibacterial activity in a continuous regime. The photosensitizers were immobilized by dissolution in chloroform, together with polystyrene, with further evaporation of the solvent, yielding thin polymeric films. Shallow reservoirs, bottom-covered with these films, were used for constructing continuous-flow photoreactors for the eradication of Gram-positive Staphylococcus aureus, Gram-negative Escherichia coli and wastewater bacteria under illumination with visible white light using a luminescent lamp at a 1.8 mW·cm-2 fluence rate. The bacterial concentration decreased by two to five orders of magnitude in separate reactors with either immobilized RB or MB, as well as in three reactors connected in series, which contained one of the photosensitizers. Bacterial eradication reached more than five orders of magnitude in two reactors connected in series, where the first reactor contained immobilized RB and the second contained immobilized MB.
KW - Antibacterial activity
KW - Immobilization
KW - Methylene blue
KW - Photodynamic antimicrobial chemotherapy
KW - Photosensitizers
KW - Polystyrene
KW - Rose bengal
UR - http://www.scopus.com/inward/record.url?scp=84906839131&partnerID=8YFLogxK
U2 - 10.3390/ijms150914984
DO - 10.3390/ijms150914984
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C2 - 25158236
AN - SCOPUS:84906839131
SN - 1661-6596
VL - 15
SP - 14984
EP - 14996
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 9
ER -