Near-infrared squaraine dyes for fluorescence enhanced surface assay

Evgenia G. Matveeva; Ewald A. Terpetschnig; Megan Stevens; Leonid Patsenker; Olga S. Kolosova; Zygmunt Gryczynski; Ignacy Gryczynski

doi:10.1016/j.dyepig.2008.04.008

Near-infrared squaraine dyes for fluorescence enhanced surface assay

Evgenia G. Matveeva, Ewald A. Terpetschnig, Megan Stevens, Leonid Patsenker, Olga S. Kolosova, Zygmunt Gryczynski, Ignacy Gryczynski

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

Original language	English
Pages (from-to)	41-46
Number of pages	6
Journal	Dyes and Pigments
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.dyepig.2008.04.008
State	Published - Jan 2009
Externally published	Yes

Keywords

Enhanced fluorescence immunoassays
Near-infrared fluorescent squaraine dyes
Sensors
Seta dyes
Silver island films
Total internal reflection

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10.1016/j.dyepig.2008.04.008

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title = "Near-infrared squaraine dyes for fluorescence enhanced surface assay",

abstract = "Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.",

keywords = "Enhanced fluorescence immunoassays, Near-infrared fluorescent squaraine dyes, Sensors, Seta dyes, Silver island films, Total internal reflection",

author = "Matveeva, {Evgenia G.} and Terpetschnig, {Ewald A.} and Megan Stevens and Leonid Patsenker and Kolosova, {Olga S.} and Zygmunt Gryczynski and Ignacy Gryczynski",

note = "Funding Information: This work was supported by Texas Emerging Technologies Fund grant and by NIH HG 004364 and NSF (DBI-0649889). ",

year = "2009",

month = jan,

doi = "10.1016/j.dyepig.2008.04.008",

language = "אנגלית",

volume = "80",

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journal = "Dyes and Pigments",

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AU - Matveeva, Evgenia G.

AU - Terpetschnig, Ewald A.

AU - Stevens, Megan

AU - Patsenker, Leonid

AU - Kolosova, Olga S.

AU - Gryczynski, Zygmunt

AU - Gryczynski, Ignacy

N1 - Funding Information: This work was supported by Texas Emerging Technologies Fund grant and by NIH HG 004364 and NSF (DBI-0649889).

PY - 2009/1

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N2 - Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

AB - Commercially available, near-infrared fluorescent squaraine dyes (Seta-635 and Seta-670) were covalently bound to antibodies and employed in surface enhanced immunoassay. From fluorescence intensity and lifetime changes determined for a surface which had been coated with silver nanoparticles as well as a non-coated glass surface, both labeled compounds exhibited a 15- to 20-fold enhancement of fluorescence on the silver-coated surface compared to that achieved on the non-coated surface. In addition, the fluorescence lifetime changed drastically for both labels in the case of silver-coated surfaces. The fluorescence signal enhancement obtained for the two dyes was greater than that previously recorded for Rhodamine Red-X and AlexaFluor-647 labels.

KW - Enhanced fluorescence immunoassays

KW - Near-infrared fluorescent squaraine dyes

KW - Sensors

KW - Seta dyes

KW - Silver island films

KW - Total internal reflection

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JO - Dyes and Pigments

JF - Dyes and Pigments

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ER -

Near-infrared squaraine dyes for fluorescence enhanced surface assay

Abstract

Keywords

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