Mix and match of KPC-2 encoding plasmids in Enterobacteriaceae-comparative genomics

Inna Chmelnitsky, Maya Shklyar, Azita Leavitt, Evgeniya Sadovsky, Shiri Navon-Venezia, Maayan Ben Dalak, Rotem Edgar, Yehuda Carmeli

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


We performed comparative sequence analysis of 3 blaKPC-2 encoding plasmids to examine evolution of these plasmids and their dissemination. We found that all of them have an IncN replicon with a newly determined IncN plasmid sequence type (ST), ST15. The 2 Klebsiella pneumoniae (KPN) plasmids also harbor an IncF2A1-B1- replicon. The blaKPC-2 is located in the Tn4401c transposon with a newly discovered mutation in the P2 promoter. Screening of the 27 additional blaKPC-2 carrying plasmids from Enterobacter cloacae, Escherichia coli (EC), and K. pneumoniae showed that: all KPN and EC plasmids are IncN plasmids belonging to ST15; 4/7 KPN and 1/6 EC plasmids contain an additional IncF2A1-B1- replicon; all Enterobacter plasmids belong to neither IncN nor IncF2A1-B1- replicon plasmids; 6/7 KPN and 2/5 EC plasmids carry the mutated P2 promoter. Study of the blaKPC-2 environment, transposon, pMLST, and Inc group suggests transposon and plasmid inter- and intra-species dissemination and evolution.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Issue number2
StatePublished - Jun 2014


  • Enterobacteriaceae
  • KPC
  • P2 promoter
  • Tn4401


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