Isolation of aggresomes and other large aggregates

Anatoli B. Meriin, Yan Wang, Michael Y. Sherman

Research output: Contribution to journalReview articlepeer-review

7 Scopus citations


Upon permanent stresses and in various diseases, small protein aggregates may accumulate in cells and cause toxicity. A recently discovered protective system transports these aggregates to the centrosome location via microtubules, to form a large agglomerate of aggregates called the aggresome. Here, we describe a newly developed method for isolating aggresomes. This principle can also be used for purification of other large structures and even organelles.

Original languageEnglish
Pages (from-to)3.38.1-3.38.9
JournalCurrent Protocols in Cell Biology
Issue numberSUPP.48
StatePublished - 2010
Externally publishedYes


  • aggregates
  • aggresome
  • subcellular fractionation
  • yeast


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