TY - JOUR
T1 - Intramuscular MAPK signaling following high volume and high intensity resistance exercise protocols in trained men
AU - Gonzalez, Adam M.
AU - Hoffman, Jay R.
AU - Townsend, Jeremy R.
AU - Jajtner, Adam R.
AU - Boone, Carleigh H.
AU - Beyer, Kyle S.
AU - Baker, Kayla M.
AU - Wells, Adam J.
AU - Mangine, Gerald T.
AU - Robinson, Edward H.
AU - Church, David D.
AU - Oliveira, Leonardo P.
AU - Fukuda, David H.
AU - Stout, Jeffrey R.
N1 - Publisher Copyright:
© 2016, Springer-Verlag Berlin Heidelberg.
PY - 2016/9/1
Y1 - 2016/9/1
N2 - Purpose: To examine the mitogen-activated protein kinase (MAPK) family of signaling proteins following typical high volume (HV) and high intensity (HI) lower body resistance exercise protocols in resistance-trained men. Methods: Ten resistance-trained men (24.7 ± 3.4 year; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) performed each resistance exercise protocol in a random, counterbalanced order. The HV protocol utilized a load of 70 % 1-RM for sets of 10–12 repetitions with a 1-min rest period length between sets and exercises. The HI protocol utilized a load of 90 % 1-RM for sets of 3–5 repetitions with a 3-min rest period length between sets and exercises. Both protocols included six sets of barbell back squats and four sets of bilateral leg press, bilateral hamstring curls, bilateral leg extensions, and seated calf raises. Fine needle muscle biopsies of the vastus lateralis were completed at baseline (BL) and 1-h post exercise (1H). Results: No significant differences over time were noted for phosphorylation of MEK1, ERK1/2, p38, MSK1, ATF2, p53, or c-Jun (p > 0.05). No significance between trial interactions was noted for phosphorylation of MAPK signaling proteins, including MEK1, ERK1/2, p38, JNK, MSK1, ATF2, STAT1, p53, c-Jun, or HSP27 (p > 0.05). However, significant time effects were observed for phosphorylation of JNK (p < 0.01), HSP27 (p < 0.01), and STAT1 (p = 0.03). Phosphorylation of JNK, HSP27, and STAT1 was significantly elevated from BL at 1H for both HV and HI. Conclusions: HV and HI lower body resistance exercise protocols appear to elicit similar MAPK activation in resistance-trained men.
AB - Purpose: To examine the mitogen-activated protein kinase (MAPK) family of signaling proteins following typical high volume (HV) and high intensity (HI) lower body resistance exercise protocols in resistance-trained men. Methods: Ten resistance-trained men (24.7 ± 3.4 year; 90.1 ± 11.3 kg; 176.0 ± 4.9 cm) performed each resistance exercise protocol in a random, counterbalanced order. The HV protocol utilized a load of 70 % 1-RM for sets of 10–12 repetitions with a 1-min rest period length between sets and exercises. The HI protocol utilized a load of 90 % 1-RM for sets of 3–5 repetitions with a 3-min rest period length between sets and exercises. Both protocols included six sets of barbell back squats and four sets of bilateral leg press, bilateral hamstring curls, bilateral leg extensions, and seated calf raises. Fine needle muscle biopsies of the vastus lateralis were completed at baseline (BL) and 1-h post exercise (1H). Results: No significant differences over time were noted for phosphorylation of MEK1, ERK1/2, p38, MSK1, ATF2, p53, or c-Jun (p > 0.05). No significance between trial interactions was noted for phosphorylation of MAPK signaling proteins, including MEK1, ERK1/2, p38, JNK, MSK1, ATF2, STAT1, p53, c-Jun, or HSP27 (p > 0.05). However, significant time effects were observed for phosphorylation of JNK (p < 0.01), HSP27 (p < 0.01), and STAT1 (p = 0.03). Phosphorylation of JNK, HSP27, and STAT1 was significantly elevated from BL at 1H for both HV and HI. Conclusions: HV and HI lower body resistance exercise protocols appear to elicit similar MAPK activation in resistance-trained men.
KW - Anabolic intramuscular signaling
KW - ERK
KW - JNK
KW - Mitogen-activated protein kinase
KW - p38
UR - http://www.scopus.com/inward/record.url?scp=84975460293&partnerID=8YFLogxK
U2 - 10.1007/s00421-016-3417-8
DO - 10.1007/s00421-016-3417-8
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C2 - 27333913
AN - SCOPUS:84975460293
SN - 1439-6319
VL - 116
SP - 1663
EP - 1670
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 9
ER -