Immobilization of antibodies onto glass wool

M. Nisnevitch, M. Kolog-Gulco, D. Trombka, B. S. Green, M. A. Firer

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The immobilization of antibodies onto solid phases in an efficient and activity-retaining form is an important goal for both research and industry. Methods have been developed for the site-directed attachment of antibodies to agarose by oxidation of the carbohydrate moieties in their Fc region. Similar attachment to silianized supports have not been as successful. Here we describe a novel combination protocol for the site-directed attachment of periodate oxidized, goat polyclonal antibodies to glass wool fibers activated with 3-aminopropyltriethoxysilane. The study demonstrates that this procedure results in effective immobilization of polyclonal antibodies that retain their antigen-binding capacity. This protocol should prove useful in the development of more efficient and effective glass-based immunosupports. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)217-223
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number2
StatePublished - 11 Feb 2000


  • Antibodies
  • Glass wool


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