Human adrenodoxin reductase: Two mRNAs encoded by a single gene on chromosome 17cen→q25 are expressed in steroidogenic tissues

S. B. Solish, J. Picado-Leonard, Y. Morel, R. W. Kuhn, T. K. Mohandas, I. Hanukoglu, W. L. Miller

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

Adrenodoxin reductase is a mitochondrial flavoprotein that receives electrons from NADPH, thus initiating the electron-transport chain serving mitochondrial cytochromes P450. We have cloned and sequenced two human adrenodoxin reductase cDNAs that differ by the presence of six additional codons in the middle of one clone. The sequence in this region indicates that these six extra codons arise by alternative splicing of the pre-mRNA. Southern blot hybridization patterns of human genomic DNA cut with four restriction enzymes indicate that the human genome has only one gene for adrenodoxin reductase. Analysis of a panel of mouse-human somatic cell hybrids localized this gene to chromosome 17cen→q25. The alternatively spliced mRNA containing the six extra codons represents 10-20% of all adrenodoxin reductase mRNA. The expression of the adrenodoxin reductase gene may be stimulated by pituitary tropic hormones acting through cAMP, but its response is quantitatively much less than the responses of P450scc and adrenodoxin.

Original languageEnglish
Pages (from-to)7104-7108
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number19
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Dive into the research topics of 'Human adrenodoxin reductase: Two mRNAs encoded by a single gene on chromosome 17cen→q25 are expressed in steroidogenic tissues'. Together they form a unique fingerprint.

Cite this