Genomics and virulence of klebsiella pneumoniae kpnu95 st1412 harboring a novel incf plasmid encoding blactx‐m‐15 and qnrs1 causing community urinary tract infection

The emergence of extended‐spectrum β‐lactamase (ESBL)‐producing multidrug resistant Klebsiella pneumoniae causing community urinary tract infections (CA‐UTI) in healthy women un-dermines effective treatment and poses a public health concern. We performed a comprehensive genomic analysis (Illumina and MinION) and virulence studies using Caenorhabditis elegans nema-todes to evaluate KpnU95, a blaCTX‐M‐15‐producing CA‐UTI K. pneumoniae strain. Whole genome sequencing identified KpnU95 as sequence type 1412 and revealed the chromosomal and plasmid-encoding resistome, virulome and persistence features. KpnU95 possess a wide virulome and caused complete C. elegans killing. The strain harbored a single novel 180.3Kb IncFIB(K) plasmid (pKpnU95), which encodes ten antibiotic resistance genes, including blaCTX‐M‐15 and qnrS1 alongside a wide persistome encoding heavy metal and UV resistance. Plasmid curing and reconstitution were used for loss and gain studies to evaluate its role on bacterial resistance, fitness and virulence. Plas-mid curing abolished the ESBL phenotype, decreased ciprofloxacin MIC and improved bacterial fitness in artificial urine accompanied with enhanced copper tolerance, without affecting bacterial virulence. Meta‐analysis supported the uniqueness of pKpnU95 and revealed plasmid‐ST1412 line-age adaptation. Overall, our findings provide translational data on a CA‐UTI K. pneumoniae ST1412 strain and demonstrates that ESBL‐encoding plasmids play key roles in multidrug resistance and in bacterial fitness and persistence.