TY - JOUR
T1 - Functionalized polysulfone as a novel and useful carrier for immunization and antibody detection
AU - Arad-Yellin, Rina
AU - Firer, Michael
AU - Kahana, Nava
AU - Green, Bernard S.
PY - 2003/1
Y1 - 2003/1
N2 - The well-known polymer, polysulfone (PS), was used, via its carboxylated derivative, as a carrier vehicle for low-molecular weight substances (haptens). The synthesis and characterization of conjugates of PS-CO2H (1) coupled to a relatively non-immunogenic aliphatic hapten (2) or an aromatic hapten (3) and various intermediates in their syntheses, are reported. Immunization with 1, 2, and 3 afforded anti-hapten antibodies and, interestingly, provided evidence for significant adjuvant activity by 1 and related substances. Further studies are called for in order to understand the mechanism of action and correlate immune activity with structure, but these initial results indicate the intriguing possibility of novel immunization methods, free of conventional, toxic adjuvants.
AB - The well-known polymer, polysulfone (PS), was used, via its carboxylated derivative, as a carrier vehicle for low-molecular weight substances (haptens). The synthesis and characterization of conjugates of PS-CO2H (1) coupled to a relatively non-immunogenic aliphatic hapten (2) or an aromatic hapten (3) and various intermediates in their syntheses, are reported. Immunization with 1, 2, and 3 afforded anti-hapten antibodies and, interestingly, provided evidence for significant adjuvant activity by 1 and related substances. Further studies are called for in order to understand the mechanism of action and correlate immune activity with structure, but these initial results indicate the intriguing possibility of novel immunization methods, free of conventional, toxic adjuvants.
KW - Adjuvant
KW - Functionalized polymer
KW - Hapten-polymer immunogen
KW - Modified polysulfone
KW - Polysulfone carboxylic acid
UR - http://www.scopus.com/inward/record.url?scp=0037224847&partnerID=8YFLogxK
U2 - 10.1016/S1381-5148(02)00178-5
DO - 10.1016/S1381-5148(02)00178-5
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AN - SCOPUS:0037224847
SN - 1381-5148
VL - 54
SP - 5
EP - 16
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
IS - 1-3
ER -