Fluorescent probes for examination of spermatozoa in cryoprotective mediums

T. S. Dyubko; M. I. Egorov; T. P. Linnik; A. P. Belonozhko; I. G. Ermolenko; O. N. Semenova; L. D. Patsenker; V. G. Pivovarenko; E. A. Povrozin

Fluorescent probes for examination of spermatozoa in cryoprotective mediums

T. S. Dyubko, M. I. Egorov, T. P. Linnik, A. P. Belonozhko, I. G. Ermolenko, O. N. Semenova, L. D. Patsenker, V. G. Pivovarenko, E. A. Povrozin

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.

Original language	English
Pages (from-to)	521-526
Number of pages	6
Journal	Tsitologiya
Volume	49
Issue number	6
State	Published - 2007
Externally published	Yes

Keywords

3-DAB
Cryoprotectants
DSM
E-176
FME
Fluorescent probes
Spermatozoa

Cite this

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title = "Fluorescent probes for examination of spermatozoa in cryoprotective mediums",

abstract = "By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.",

keywords = "3-DAB, Cryoprotectants, DSM, E-176, FME, Fluorescent probes, Spermatozoa",

author = "Dyubko, {T. S.} and Egorov, {M. I.} and Linnik, {T. P.} and Belonozhko, {A. P.} and Ermolenko, {I. G.} and Semenova, {O. N.} and Patsenker, {L. D.} and Pivovarenko, {V. G.} and Povrozin, {E. A.}",

year = "2007",

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volume = "49",

pages = "521--526",

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TY - JOUR

T1 - Fluorescent probes for examination of spermatozoa in cryoprotective mediums

AU - Dyubko, T. S.

AU - Egorov, M. I.

AU - Linnik, T. P.

AU - Belonozhko, A. P.

AU - Ermolenko, I. G.

AU - Semenova, O. N.

AU - Patsenker, L. D.

AU - Pivovarenko, V. G.

AU - Povrozin, E. A.

PY - 2007

Y1 - 2007

N2 - By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.

AB - By fluorescent spectroscopy and microscopy methods the possibility of fluorescent probes DSM, E-176, 3-DAB and FME application for study of cryoprotective agents' influence on the dog spermatozoa are investigated. It is established that FME and 3-DAD dyes are suitable for the posed problem solving, and the DSM and E-176 probes have restrictions owing to enough strong fluorescence from cryoprotectant solutions. It is shown that the fluorescent probes investigated influence the cells motility to different degree. The perspectives of investigated dyes application for study of cryoprotective agent' finfluence on spermatozoa are considered.

KW - 3-DAB

KW - Cryoprotectants

KW - DSM

KW - E-176

KW - FME

KW - Fluorescent probes

KW - Spermatozoa

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JO - Tsitologiya

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Fluorescent probes for examination of spermatozoa in cryoprotective mediums

Abstract

Keywords

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