Fluorescence attenuated by a thick scattering medium: Theory, simulations and experiments

Yitzchak Weber, Hamootal Duadi, Pavitra Sokke Rudraiah, Inbar Yariv, Gilad Yahav, Dror Fixler, Rinat Ankri

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Fluorescence-based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging. In this article, we present a diffusion model, based on an existing master–slave model, of isotropic point sources imbedded in a scattering slab, representing fluorophores within a tissue. The model was compared with Monte Carlo simulations and measurements of a fluorescent slide measured through tissue-like phantoms with different reduced scattering coefficients (0.5–2.5 mm−1) and thicknesses (0.5–5 mm). Results show a good correlation between our suggested theory, simulations and experiments; while the fluorescence intensity decays as the slab's scattering and thickness increase, the decay rate decreases as the reduced scattering coefficient increases in a counterintuitive manner, suggesting fewer fluorescence artifacts from deep within the tissue in highly scattering media.

Original languageEnglish
Article numbere202300045
JournalJournal of Biophotonics
Issue number6
StatePublished - Jun 2023


  • fluorescence imaging
  • fluorescence intensity
  • photon diffusion


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