TY - JOUR
T1 - Fluorescence attenuated by a thick scattering medium
T2 - Theory, simulations and experiments
AU - Weber, Yitzchak
AU - Duadi, Hamootal
AU - Rudraiah, Pavitra Sokke
AU - Yariv, Inbar
AU - Yahav, Gilad
AU - Fixler, Dror
AU - Ankri, Rinat
N1 - Publisher Copyright:
© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
PY - 2023/6
Y1 - 2023/6
N2 - Fluorescence-based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging. In this article, we present a diffusion model, based on an existing master–slave model, of isotropic point sources imbedded in a scattering slab, representing fluorophores within a tissue. The model was compared with Monte Carlo simulations and measurements of a fluorescent slide measured through tissue-like phantoms with different reduced scattering coefficients (0.5–2.5 mm−1) and thicknesses (0.5–5 mm). Results show a good correlation between our suggested theory, simulations and experiments; while the fluorescence intensity decays as the slab's scattering and thickness increase, the decay rate decreases as the reduced scattering coefficient increases in a counterintuitive manner, suggesting fewer fluorescence artifacts from deep within the tissue in highly scattering media.
AB - Fluorescence-based imaging has an enormous impact on our understanding of biological systems. However, in vivo fluorescence imaging is greatly influenced by tissue scattering. A better understanding of this dependence can improve the potential of noninvasive in vivo fluorescence imaging. In this article, we present a diffusion model, based on an existing master–slave model, of isotropic point sources imbedded in a scattering slab, representing fluorophores within a tissue. The model was compared with Monte Carlo simulations and measurements of a fluorescent slide measured through tissue-like phantoms with different reduced scattering coefficients (0.5–2.5 mm−1) and thicknesses (0.5–5 mm). Results show a good correlation between our suggested theory, simulations and experiments; while the fluorescence intensity decays as the slab's scattering and thickness increase, the decay rate decreases as the reduced scattering coefficient increases in a counterintuitive manner, suggesting fewer fluorescence artifacts from deep within the tissue in highly scattering media.
KW - fluorescence imaging
KW - fluorescence intensity
KW - photon diffusion
UR - http://www.scopus.com/inward/record.url?scp=85150928875&partnerID=8YFLogxK
U2 - 10.1002/jbio.202300045
DO - 10.1002/jbio.202300045
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AN - SCOPUS:85150928875
SN - 1864-063X
VL - 16
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 6
M1 - e202300045
ER -