TY - JOUR
T1 - Examining protein-lipid interactions in model systems with a new squarylium fluorescent dye
AU - Ioffe, Valeriya M.
AU - Gorbenko, Galyna P.
AU - Tatarets, Anatoliy L.
AU - Patsenker, Leonid D.
AU - Terpechnig, Ewald A.
PY - 2006/7
Y1 - 2006/7
N2 - The applicability of newly synthesized squarylium dye Sq to probing the changes in physical characteristics of lipid bilayer on the formation of protein-lipid complexes has been evaluated. Lipid vesicles composed of zwitterionic phospholipid phosphatidylcholine (PC) and its mixtures with positively charged detergent cetyltrimethylammonium bromide (CTAB), anionic phospholipid cardiolipin (CL), and cholesterol (Chol) were employed as lipid component of model membrane systems while protein constituent was represented by lysozyme (Lz). Fluorescence intensity of Sq was found to decrease on Lz association with lipid bilayer. This effect was observed in all kinds of model systems suggesting that Sq is sensitive to modification of lipid bilayer physical properties on hydrophobic protein-lipid interactions. It was found that Sq spectral response to variations in Chol content depends on relative contributions of electrostatic and hydrophobic components of Lz-membrane binding.
AB - The applicability of newly synthesized squarylium dye Sq to probing the changes in physical characteristics of lipid bilayer on the formation of protein-lipid complexes has been evaluated. Lipid vesicles composed of zwitterionic phospholipid phosphatidylcholine (PC) and its mixtures with positively charged detergent cetyltrimethylammonium bromide (CTAB), anionic phospholipid cardiolipin (CL), and cholesterol (Chol) were employed as lipid component of model membrane systems while protein constituent was represented by lysozyme (Lz). Fluorescence intensity of Sq was found to decrease on Lz association with lipid bilayer. This effect was observed in all kinds of model systems suggesting that Sq is sensitive to modification of lipid bilayer physical properties on hydrophobic protein-lipid interactions. It was found that Sq spectral response to variations in Chol content depends on relative contributions of electrostatic and hydrophobic components of Lz-membrane binding.
KW - Liposomes
KW - Lysozyme
KW - Protein-lipid interactions
KW - Squarylium dye
UR - http://www.scopus.com/inward/record.url?scp=33745923547&partnerID=8YFLogxK
U2 - 10.1007/s10895-006-0092-x
DO - 10.1007/s10895-006-0092-x
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C2 - 16794868
AN - SCOPUS:33745923547
SN - 1053-0509
VL - 16
SP - 547
EP - 554
JO - Journal of Fluorescence
JF - Journal of Fluorescence
IS - 4
ER -