Elimination of non-specific binding in Western blots from non-reducing gels

Israel Hanukoglu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The reaction of some antibodies with Western blots of protein shows strong non-specific binding especially at a region that corresponds to about 70-90 kDa. This binding is independent of protein concentration. Further analysis indicated that the factor responsible for the non-specific background binding 2-mercaptoethanol in the gel sample buffer. Gel electrophoresis of total tissue homogenates in the absence of this reducing agent resulted in dramatic elimination of the non-specific background binding without affecting the mobility of the two proteins we studied.

Original languageEnglish
Pages (from-to)65-68
Number of pages4
JournalJournal of Biochemical and Biophysical Methods
Issue number1
StatePublished - Jun 1990
Externally publishedYes


  • Cytochrome P450
  • Electrophoresis


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