Effect of cholesterol depletion on the electrical activity of rat heart myocytes in culture

Yonathan Hasin; Yakhin Shimoni; Olga Stein; Yechezkiel Stein

doi:10.1016/0022-2828(80)90098-X

Effect of cholesterol depletion on the electrical activity of rat heart myocytes in culture

Yonathan Hasin, Yakhin Shimoni, Olga Stein, Yechezkiel Stein

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The cholesterol content of biological membranes is known to affect their fluidity and their permeability. In the present work we examine the effects of cholesterol depletion on the electrical activity of cultured rat cardiac cells. Electrical activity was recorded with intracellular electrodes in normal and cholesterol-depleted cardiac cells. Cholesterol depletion of 25 to 42% (mean 33%) was obtained by exposure of the cells to high density apolipoprotein-sphingomyelin mixtures. Cholesterol-depleted cells manifested a significantly faster rate of depolarization than control cells; their spontaneous activity was more resistant to [Na⁺] or [Ca²⁺] deprivation, low pH, verapamil and tetrodotoxin. The present findings are consistent with an increase in calcium and sodium influxes during depolarization in cholesterol-depleted rat heart cells. The present findings are consistent with the hypothesis that cholesterol depletion in rat heart cells causes an increase in calcium and sodium influxes during depolarization.

Original language	English
Pages (from-to)	675-683
Number of pages	9
Journal	Journal of Molecular and Cellular Cardiology
Volume	12
Issue number	7
DOIs	https://doi.org/10.1016/0022-2828(80)90098-X
State	Published - Jul 1980
Externally published	Yes

Keywords

Action potential
Cultured heart cells
Ion channels
Membrane cholesterol content
Membrane permeability
Slow cardiac fibers

Access to Document

10.1016/0022-2828(80)90098-X

Cite this

@article{bb7357590ed2474c9cd9002ebd17216a,

title = "Effect of cholesterol depletion on the electrical activity of rat heart myocytes in culture",

abstract = "The cholesterol content of biological membranes is known to affect their fluidity and their permeability. In the present work we examine the effects of cholesterol depletion on the electrical activity of cultured rat cardiac cells. Electrical activity was recorded with intracellular electrodes in normal and cholesterol-depleted cardiac cells. Cholesterol depletion of 25 to 42% (mean 33%) was obtained by exposure of the cells to high density apolipoprotein-sphingomyelin mixtures. Cholesterol-depleted cells manifested a significantly faster rate of depolarization than control cells; their spontaneous activity was more resistant to [Na+] or [Ca2+] deprivation, low pH, verapamil and tetrodotoxin. The present findings are consistent with an increase in calcium and sodium influxes during depolarization in cholesterol-depleted rat heart cells. The present findings are consistent with the hypothesis that cholesterol depletion in rat heart cells causes an increase in calcium and sodium influxes during depolarization.",

keywords = "Action potential, Cultured heart cells, Ion channels, Membrane cholesterol content, Membrane permeability, Slow cardiac fibers",

author = "Yonathan Hasin and Yakhin Shimoni and Olga Stein and Yechezkiel Stein",

note = "Funding Information: The excellent assistance of Mrs A. Mendeles and Mrs Ch. Cobin is gratefully acknowledged. The authors would like to express special thanks to Dr R. Rahamimoff for his invaluable help in the critical evaluation of the research project and the preparation of the manuscript. This investigation was supported in part by a grant from the United States-Israel Binational Science Foundation and from the Delegation Generale a la Recherche Scientifique et Technique the French Government, and by a grant from the Joint Research Foundation of The Hebrew University and Hadassah to Dr Y. Hasin. Dr 0. Stein and Dr. Y. Stein are Established Investigators of the Ministry of Health.",

year = "1980",

month = jul,

doi = "10.1016/0022-2828(80)90098-X",

language = "אנגלית",

volume = "12",

pages = "675--683",

journal = "Journal of Molecular and Cellular Cardiology",

issn = "0022-2828",

publisher = "Academic Press",

number = "7",

}

TY - JOUR

T1 - Effect of cholesterol depletion on the electrical activity of rat heart myocytes in culture

AU - Hasin, Yonathan

AU - Shimoni, Yakhin

AU - Stein, Olga

AU - Stein, Yechezkiel

N1 - Funding Information: The excellent assistance of Mrs A. Mendeles and Mrs Ch. Cobin is gratefully acknowledged. The authors would like to express special thanks to Dr R. Rahamimoff for his invaluable help in the critical evaluation of the research project and the preparation of the manuscript. This investigation was supported in part by a grant from the United States-Israel Binational Science Foundation and from the Delegation Generale a la Recherche Scientifique et Technique the French Government, and by a grant from the Joint Research Foundation of The Hebrew University and Hadassah to Dr Y. Hasin. Dr 0. Stein and Dr. Y. Stein are Established Investigators of the Ministry of Health.

PY - 1980/7

Y1 - 1980/7

N2 - The cholesterol content of biological membranes is known to affect their fluidity and their permeability. In the present work we examine the effects of cholesterol depletion on the electrical activity of cultured rat cardiac cells. Electrical activity was recorded with intracellular electrodes in normal and cholesterol-depleted cardiac cells. Cholesterol depletion of 25 to 42% (mean 33%) was obtained by exposure of the cells to high density apolipoprotein-sphingomyelin mixtures. Cholesterol-depleted cells manifested a significantly faster rate of depolarization than control cells; their spontaneous activity was more resistant to [Na+] or [Ca2+] deprivation, low pH, verapamil and tetrodotoxin. The present findings are consistent with an increase in calcium and sodium influxes during depolarization in cholesterol-depleted rat heart cells. The present findings are consistent with the hypothesis that cholesterol depletion in rat heart cells causes an increase in calcium and sodium influxes during depolarization.

AB - The cholesterol content of biological membranes is known to affect their fluidity and their permeability. In the present work we examine the effects of cholesterol depletion on the electrical activity of cultured rat cardiac cells. Electrical activity was recorded with intracellular electrodes in normal and cholesterol-depleted cardiac cells. Cholesterol depletion of 25 to 42% (mean 33%) was obtained by exposure of the cells to high density apolipoprotein-sphingomyelin mixtures. Cholesterol-depleted cells manifested a significantly faster rate of depolarization than control cells; their spontaneous activity was more resistant to [Na+] or [Ca2+] deprivation, low pH, verapamil and tetrodotoxin. The present findings are consistent with an increase in calcium and sodium influxes during depolarization in cholesterol-depleted rat heart cells. The present findings are consistent with the hypothesis that cholesterol depletion in rat heart cells causes an increase in calcium and sodium influxes during depolarization.

KW - Action potential

KW - Cultured heart cells

KW - Ion channels

KW - Membrane cholesterol content

KW - Membrane permeability

KW - Slow cardiac fibers

UR - http://www.scopus.com/inward/record.url?scp=0018926585&partnerID=8YFLogxK

U2 - 10.1016/0022-2828(80)90098-X

DO - 10.1016/0022-2828(80)90098-X

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 7411645

AN - SCOPUS:0018926585

SN - 0022-2828

VL - 12

SP - 675

EP - 683

JO - Journal of Molecular and Cellular Cardiology

JF - Journal of Molecular and Cellular Cardiology

IS - 7

ER -

Effect of cholesterol depletion on the electrical activity of rat heart myocytes in culture

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this