TY - JOUR
T1 - Double-modified albumins as a tool for antibody purification
AU - Patchornik, Guy
PY - 2007/6/10
Y1 - 2007/6/10
N2 - A general approach for anti-hapten antibody purification utilizing double-modified albumins is presented. Purification is based on simultaneous modification of an albumin with a hapten (e.g. fluorescein) and desthiobiotin. Three distinct albumins (BSA, HSA and ovalbumin) were modified accordingly and evaluated for their ability to purify the anti-fluorescein mAb from a mixture of commercial preparation and an E. coli cell lysate. The recovered mAb was obtained at relatively high purity (88-95%), in a wide range of target concentrations (0.66-0.02 mg/ml) within a total purification time of ∼ 20 min. Substantial increase in the contamination background did not affect purity.
AB - A general approach for anti-hapten antibody purification utilizing double-modified albumins is presented. Purification is based on simultaneous modification of an albumin with a hapten (e.g. fluorescein) and desthiobiotin. Three distinct albumins (BSA, HSA and ovalbumin) were modified accordingly and evaluated for their ability to purify the anti-fluorescein mAb from a mixture of commercial preparation and an E. coli cell lysate. The recovered mAb was obtained at relatively high purity (88-95%), in a wide range of target concentrations (0.66-0.02 mg/ml) within a total purification time of ∼ 20 min. Substantial increase in the contamination background did not affect purity.
KW - Affinity chromatography
KW - Affinity sinking
KW - Antibody purification
KW - Avidin
KW - Desthiobiotin
KW - Streptavidin
UR - http://www.scopus.com/inward/record.url?scp=34247593752&partnerID=8YFLogxK
U2 - 10.1016/j.jbbm.2007.01.012
DO - 10.1016/j.jbbm.2007.01.012
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C2 - 17324468
AN - SCOPUS:34247593752
SN - 0165-022X
VL - 70
SP - 671
EP - 673
JO - Journal of Biochemical and Biophysical Methods
JF - Journal of Biochemical and Biophysical Methods
IS - 4
ER -