Development of sensitive methods for the detection of myobacteria by DNA probes

Galia Rahav; Shlomo Sela; Hervé Bercovier

doi:10.1016/0378-1097(90)90339-R

Development of sensitive methods for the detection of myobacteria by DNA probes

Galia Rahav, Shlomo Sela, Hervé Bercovier

Research output: Contribution to journal › Article › peer-review

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Abstract

A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

Original language	English
Pages (from-to)	29-33
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	72
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1097(90)90339-R
State	Published - Oct 1990
Externally published	Yes

Keywords

DNA probes
DNA-DNA hybridization
Mycobacteria
Sonication

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10.1016/0378-1097(90)90339-R

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title = "Development of sensitive methods for the detection of myobacteria by DNA probes",

abstract = "A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.",

keywords = "DNA probes, DNA-DNA hybridization, Mycobacteria, Sonication",

author = "Galia Rahav and Shlomo Sela and Herv{\'e} Bercovier",

note = "Funding Information: We would like to thank R. David-Pour for her excellent technical assistance. This work was in part funded by a grant from the Hebrew University and Hadassah Hospital common research fund.",

year = "1990",

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AU - Rahav, Galia

AU - Sela, Shlomo

AU - Bercovier, Hervé

N1 - Funding Information: We would like to thank R. David-Pour for her excellent technical assistance. This work was in part funded by a grant from the Hebrew University and Hadassah Hospital common research fund.

PY - 1990/10

Y1 - 1990/10

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AB - A sensitive method allowing the detection of myobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.

KW - DNA probes

KW - DNA-DNA hybridization

KW - Mycobacteria

KW - Sonication

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Development of sensitive methods for the detection of myobacteria by DNA probes

Abstract

Keywords

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