Determination of coproporphyrinogen III oxidase activity in bacteria

Rivka Mamet; Ram Scharf; Nathan Keller; Yoram Zimmels; Nili Schoenfeld

doi:10.1016/0167-7012(94)90067-1

Determination of coproporphyrinogen III oxidase activity in bacteria

Rivka Mamet, Ram Scharf, Nathan Keller, Yoram Zimmels, Nili Schoenfeld

Research output: Contribution to journal › Article › peer-review

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Abstract

A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

Original language	English
Pages (from-to)	173-178
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	19
Issue number	3
DOIs	https://doi.org/10.1016/0167-7012(94)90067-1
State	Published - Mar 1994
Externally published	Yes

Keywords

Coproporphyrinogen III oxidase
E. coli
HPLC

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10.1016/0167-7012(94)90067-1

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title = "Determination of coproporphyrinogen III oxidase activity in bacteria",

abstract = "A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.",

keywords = "Coproporphyrinogen III oxidase, E. coli, HPLC",

author = "Rivka Mamet and Ram Scharf and Nathan Keller and Yoram Zimmels and Nili Schoenfeld",

year = "1994",

month = mar,

doi = "10.1016/0167-7012(94)90067-1",

language = "אנגלית",

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T1 - Determination of coproporphyrinogen III oxidase activity in bacteria

AU - Mamet, Rivka

AU - Scharf, Ram

AU - Keller, Nathan

AU - Zimmels, Yoram

AU - Schoenfeld, Nili

PY - 1994/3

Y1 - 1994/3

N2 - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

AB - A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

KW - Coproporphyrinogen III oxidase

KW - E. coli

KW - HPLC

UR - https://www.scopus.com/pages/publications/0028348848

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DO - 10.1016/0167-7012(94)90067-1

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AN - SCOPUS:0028348848

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Determination of coproporphyrinogen III oxidase activity in bacteria

Abstract

Keywords

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