Determination of coproporphyrinogen III oxidase activity in bacteria

Rivka Mamet, Ram Scharf, Nathan Keller, Yoram Zimmels, Nili Schoenfeld

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3 Scopus citations


A rapid HPLC method for determining protoporphyrin is employed in the procedure described for measuring coproporphyrinogen III oxidase (CO) activity in bacteria. The activity was found to be linear with protein up to 300 μg/assay and with incubation time up to 2 h. CO activities measured in Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae were found to be 237, 128 and 22 pmol protoporphyrin/mg protein/h, respectively.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalJournal of Microbiological Methods
Issue number3
StatePublished - Mar 1994
Externally publishedYes


  • Coproporphyrinogen III oxidase
  • E. coli
  • HPLC


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