TY - JOUR
T1 - Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel
AU - Chmelnitsky, Inna
AU - Hermesh, Orit
AU - Navon-Venezia, Shiri
AU - Strahilevitz, Jacob
AU - Carmeli, Yehuda
N1 - Funding Information:
This work was supported by the Research Fund of Chief Scientist Office, Ministry of Health (Jerusalem, Israel), and by the Morasha 1833/07 grant from the Israel Science Foundation awarded to Dr Jacob Strahilevitz.
PY - 2009
Y1 - 2009
N2 - Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.
AB - Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.
KW - Carbapenem resistance
KW - Plasmids
KW - Quinolone resistance
KW - β-lactamases
UR - http://www.scopus.com/inward/record.url?scp=70349467522&partnerID=8YFLogxK
U2 - 10.1093/jac/dkp272
DO - 10.1093/jac/dkp272
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C2 - 19656782
AN - SCOPUS:70349467522
SN - 0305-7453
VL - 64
SP - 718
EP - 722
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
IS - 4
ER -