Detection of aac(6′)-Ib-cr in KPC-producing Klebsiella pneumoniae isolates from Tel Aviv, Israel

Inna Chmelnitsky, Orit Hermesh, Shiri Navon-Venezia, Jacob Strahilevitz, Yehuda Carmeli

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Objectives: We aimed to evaluate the occurrence and characteristics of plasmid-mediated quinolone resistance (PMQR) genes in KPC-producing (KPC-P) Klebsiella pneumoniae (Kpn) isolates in Tel Aviv Medical Center, Israel. Methods: Forty-seven KPC-P Kpn isolates were studied. Antibiotic susceptibilities were determined by Vitek 2, Etest or agar dilution. β-Lactamases and PMQR determinants were detected by PCR. For plasmid characterization, transformation, transconjugation, restriction mapping and Southern blot analysis were performed. Results: Six out of 47 (13%) KPC-P isolates carried aac(6′)-Ib-cr. Acquisition of aac(6′)-Ib-cr-encoding plasmids increased the MIC of ciprofloxacin by 2-fold. In five of six KPC-P isolates, aac(6′)-Ib-cr and blaKPC-2 were encoded on the same plasmid. Conclusions: The most prevalent PMQR gene in the studied KPC-PK. pneumoniae isolates is aac(6′)-Ib-cr. The co-existence of PMQR genes with KPC on the same plasmid poses a serious epidemiological, clinical and public-health threat.

Original languageEnglish
Pages (from-to)718-722
Number of pages5
JournalJournal of Antimicrobial Chemotherapy
Volume64
Issue number4
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Carbapenem resistance
  • Plasmids
  • Quinolone resistance
  • β-lactamases

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