Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

J. R. Hutson; K. Aleksa; F. Pragst; G. Koren

doi:10.1016/j.jchromb.2008.10.056

Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

J. R. Hutson, K. Aleksa, F. Pragst, G. Koren

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

Original language	English
Pages (from-to)	8-12
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2008.10.056
State	Published - 1 Jan 2009
Externally published	Yes

Keywords

Biomarker
Fatty acid ethyl esters
Gas chromatography
Headspace-solid-phase microextraction
Meconium

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10.1016/j.jchromb.2008.10.056

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title = "Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry",

abstract = "Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.",

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author = "Hutson, {J. R.} and K. Aleksa and F. Pragst and G. Koren",

note = "Funding Information: JRH is supported by the University of Toronto{\textquoteright}s Comprehensive Research Experience for Medical Students Summer Studentship and a CIHR MD/PhD Studentship. GK is the holder of the Ivey Chair in Molecular Toxicology, The Department of Medicine, University of Western Ontario.",

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volume = "877",

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Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry. / Hutson, J. R.; Aleksa, K.; Pragst, F. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 1-2, 01.01.2009, p. 8-12.

Research output: Contribution to journal › Article › peer-review

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AU - Hutson, J. R.

AU - Aleksa, K.

AU - Pragst, F.

AU - Koren, G.

N1 - Funding Information: JRH is supported by the University of Toronto’s Comprehensive Research Experience for Medical Students Summer Studentship and a CIHR MD/PhD Studentship. GK is the holder of the Ivey Chair in Molecular Toxicology, The Department of Medicine, University of Western Ontario.

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AB - Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

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KW - Fatty acid ethyl esters

KW - Gas chromatography

KW - Headspace-solid-phase microextraction

KW - Meconium

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Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

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Keywords

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