Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry

J. R. Hutson, K. Aleksa, F. Pragst, G. Koren

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Meconium fatty acid ethyl esters (FAEEs) are currently used as biomarkers to detect heavy prenatal alcohol exposure. We introduce a novel technique to quantify FAEEs in meconium using headspace-solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). This method improves on previous approaches by decreasing sample preparation time, eliminating the need for organic solvents, and reducing the required sample size. Using 50 mg of meconium, the detection limits of FAEEs ranged from 0.05 to 0.16 nmol/g and had good reproducibility making it ideal for routine analysis of clinical samples.

Original languageEnglish
Pages (from-to)8-12
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number1-2
DOIs
StatePublished - 1 Jan 2009
Externally publishedYes

Keywords

  • Biomarker
  • Fatty acid ethyl esters
  • Gas chromatography
  • Headspace-solid-phase microextraction
  • Meconium

Fingerprint

Dive into the research topics of 'Detection and quantification of fatty acid ethyl esters in meconium by headspace-solid-phase microextraction and gas chromatography-mass spectrometry'. Together they form a unique fingerprint.

Cite this