Current production in a microbial fuel cell using a pure culture of Cupriavidus basilensis growing in acetate or phenol as a carbon source

Hen Friman, Alex Schechter, Yulia Ioffe, Yeshayahu Nitzan, Rivka Cahan

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77 Scopus citations

Abstract

A microbial fuel cell (MFC) was operated with a pure culture of Cupriavidus basilensis bacterial cells growing in the anode compartment in a defined medium containing acetate or phenol. Operating this mediator-less MFC under a constant external resistor of 1kΩ with acetate or phenol led to current generation of 902 and 310mAm-2 respectively. In the MFC which was operated using acetate or phenol, the current density measured from the plankton bacterial cells with a fresh electrode was 125 and 109mAm-2, respectively, whereas the current obtained with biofilm-covered electrodes in sterile medium was 541 and 228mAm-2 respectively. After 72h in the MFC, 86% of the initial phenol concentration was removed, while only 64% was removed after the same time in the control MFC which was held at an open circuit potential (OCP). Furthermore, SEM and confocal microscopy analyses demonstrated a developed biofilm with a live C.basilensis population. In conclusion, in this study we demonstrated, for the first time, use of C.basilensis facultative aerobe bacterial cells in a MFC using acetate or phenol as the sole carbon source which led to electricity generation.

Original languageEnglish
Pages (from-to)425-434
Number of pages10
JournalMicrobial Biotechnology
Volume6
Issue number4
DOIs
StatePublished - Jul 2013

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