Contrasting effects on bone formation and on fracture healing of cholecalciferol and of 1 α-hydroxycholecalciferol

S. Dekel, A. Ornoy, E. Sekeles, D. Noff, S. Edelstein

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21 Scopus citations


Experimental fractures were performed on tibiae of vitamin D-depleted chicks. The chicks were divided into three groups; they were treated daily with (a) cholecalciferol or (b) 1α-hydroxycholecalciferol (1α(OH)D3), or (c) they received no treatment. Microscopic examination of the calluses formed at the fractured sites and of the proximal tibiae of the contralateral legs showed that treatment with 1α(OH)D3 failed to heal most of the rachitic signs seen in the nontreated chicks, despite normal plasma concentrations of calcium and of inorganic phosphorus. In a second experiment, experimental fractures were performed on tibiae of chicks that were fed a vitamin D-deficient diet, but were dosed continuously with radioactive cholecalciferol. Analysis of cholecalciferol metabolites in the callus tissue showed perferential accumulation of 25-hydroxycholecalciferol (25(OH)D3) and of 24,25-dihydroxycholecalciferol (24,25(OH)2D3). Very little 1α, 25-dihydroxycholecalciferol (1α,25(OH)2D3) was detected in bones or in calluses. Based on the data obtained, the following conclusions were drawn: (a) that cholecalciferol is directly involved in bone formation; and (b) that 1α, 25(OH)2D3 is not the sole metabolite of cholecalciferol involved in this process.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalCalcified Tissue International
Issue number1
StatePublished - Dec 1979
Externally publishedYes


  • 1α-Hydroxycholecalciferol
  • Bone formation
  • Cholecalciferol
  • Fractures


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